Nanoprobe-based genetic testing

Nanoprobe-based genetic testing

• Yanbing Zu^a, 
• Min-Han Tan^a, 
• Balram Chowbay^b, c, d, 
• Soo Chin Lee^e, f, 
• Huiling Yap^e, 
• Ming Ta Michael Lee^g,h, 
• Liang-Suei Lu^g, 
• Chun-Ping Chang^g, 
• Jackie Y. Ying^a, , 

Highlights

•

We have developed a nanoprobe-based colorimetric method for single-nucleotide polymorphism (SNP) genotyping.

•

The only equipment involved in the assay is a standard thermal cycler, allowing for easy deployment and cost-effective genetic testing.

•

The method is successfully used to gauge three SNPs that are associated with warfarin sensitivity.

•

The warfarin sensitivity assay kit has been validated with 249 human genomic DNA samples in four independent institutes.

---

Summary

Plasmonic nanoprobes functionalized with morpholino oligos have been developed for genotyping of single-nucleotide polymorphisms (SNPs) based on colorimetric signals that could be easily visualized. The assay was conducted with a standard thermal cycler that would be available in any molecular diagnostic laboratory, facilitating assay deployment and allowing for cost-effective genetic testing with rapid turnaround time. Specifically, a warfarin sensitivity testing kit gauging three SNPs has been created and validated with 249 human genomic DNA samples in four independent institutes, demonstrating the robustness of our new platform.