domingo, 8 de junio de 2014

Nanoprobe-based genetic testing

Nanoprobe-based genetic testing

Cover image

Nanoprobe-based genetic testing


We have developed a nanoprobe-based colorimetric method for single-nucleotide polymorphism (SNP) genotyping.
The only equipment involved in the assay is a standard thermal cycler, allowing for easy deployment and cost-effective genetic testing.
The method is successfully used to gauge three SNPs that are associated with warfarin sensitivity.
The warfarin sensitivity assay kit has been validated with 249 human genomic DNA samples in four independent institutes.


Plasmonic nanoprobes functionalized with morpholino oligos have been developed for genotyping of single-nucleotide polymorphisms (SNPs) based on colorimetric signals that could be easily visualized. The assay was conducted with a standard thermal cycler that would be available in any molecular diagnostic laboratory, facilitating assay deployment and allowing for cost-effective genetic testing with rapid turnaround time. Specifically, a warfarin sensitivity testing kit gauging three SNPs has been created and validated with 249 human genomic DNA samples in four independent institutes, demonstrating the robustness of our new platform.

No hay comentarios:

Publicar un comentario