J Virol Methods. 2013 Dec 7. pii: S0166-0934(13)00488-6. doi: 10.1016/j.jviromet.2013.11.017. [Epub ahead of print]
Identification of vaccine-derived polioviruses using dual-stage real-time RT-PCR.
Kilpatrick DR1, Ching K2, Iber J2, Chen Q2, Yang SJ2, De L2, Williams AJ2, Mandelbaum M2, Sun H2, Oberste MS2, Kew OM2.
Vaccine-derived polioviruses (VDPVs) are associated with polio outbreaks and prolonged infections in individuals with primary immunodeficiencies. VDPV-specific PCR assays for each of the three Sabin oral poliovirus vaccine (OPV) strains were developed, targeting sequences within the VP1 capsid region that are selected for during replication of OPV in the human intestine. Over 2400 Sabin-related isolates and identified 755 VDPVs were screened. Sensitivity of all assays was 100%, while specificity was 100% for serotypes 1 and 3, and 76% for serotype 2. The assays permit rapid, sensitive identification of OPV-related viruses and flag programmatically important isolates for further characterization by genomic sequencing.
Published by Elsevier B.V.
Poliovirus, Real-time RT-PCR, VDPV, Vaccine-derived poliovirus
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