Evaluation of mailed pediatric buccal cytobrushes ... [Birth Defects Res A Clin Mol Teratol. 2011] - PubMed result

Birth Defects Res A Clin Mol Teratol. 2011 May 31. doi: 10.1002/bdra.20829. [Epub ahead of print]
Evaluation of mailed pediatric buccal cytobrushes for use in a case-control study of birth defects.
Gallagher ML, Sturchio C, Smith A, Koontz D, Jenkins MM, Honein MA, Rasmussen SA.
Source
National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia. MGallagher1@cdc.gov.

Abstract
BACKGROUND: Buccal cell collection is a convenient DNA collection method; however, little attention has been given to the quality of DNA obtained from pediatric populations. The purpose of this study was to determine the effect of a modified cytobrush collection method on the yield and quality of infant buccal DNA collected as part of a population-based case-control study of birth defects. METHODS Cytobrushes were collected from infants, mothers, and fathers using a standard collection method in 1997 to 2003 and a modified protocol that allows air-drying of the cytobrushes after collection from 2003 to the present. Yield and quality of DNA from 1057 cytobrushes was assessed by quantitative PCR and short tandem repeat (STR) genotyping, respectively. RESULTS Air-dried cytobrushes from infants had higher median DNA yields (1300 ng) and STR completion rates (99.5%) than standard collection method cytobrushes (60 ng and 59.5%, respectively). A subset of DNA aliquots was genotyped for six single nucleotide polymorphisms (SNPs). Aliquots from both collection methods that passed the quality protocol (DNA concentration >1 ng/μl, and successful amplification of ≥1 STR) had high genotype completion rates (99-100%). The median DNA yield following whole genome amplification was more than twofold higher for air-dried than standard collection specimens (p < 0.001). CONCLUSION Yield and quality of buccal DNA collected from infants are improved by using a method that incorporates air-drying; however, DNA collected by both methods is suitable for genotyping if stringent quality control procedures are instituted. These findings may be helpful for future epidemiologic studies of birth defects and other adverse pediatric outcomes. Birth Defects Research (Part A) 2011. © 2011 Wiley-Liss, Inc.

Copyright © 2011 Wiley-Liss, Inc.

PMID:21630425[PubMed - as supplied by publisher]
Evaluation of mailed pediatric buccal cytobrushes ... [Birth Defects Res A Clin Mol Teratol. 2011] - PubMed result