CRISPRz

CRISPR/Cas9 has emerged as a versatile genome editing tool and is being used in a wide variety of cell lines and organisms including zebrafish. The Burgess lab has developed a streamlined pipeline for generating gene knockouts using CRISPR/Cas9 in a high-throughput manner.

CRISPRz is a curated database of validated CRISPR targets in zebrafish maintained by Shawn Burgess' lab at NHGRI/NIH. The database contains target sites from the Burgess lab's large-scale mutagenesis project, as well as those reported in the literature. A search form allows users to search by various identifiers in order to determine if a validated CRISPR target exists for a gene of interest.

The CRISPRz site also hosts methods and protocols developed by the Burgess lab, software for identifying indels generated by CRISPR/Cas9 (ampliconDIVider), and links to CRISPR target tracks on the UCSC Genome browser.

We welcome submissions to CRISPRz. If you wish to add CRISPR target activity data from your lab, please use fill out this template and email to: Gaurav Varshney at Gaurav-varshney@omrf.org

To get CRISPR targets tracks at the UCSC Genome Browser, please click HERE.

Sept 24, 2018 data release (genome assembly danRer11):

Current CRISPR target sequences: 1697. Number of Sources: 13

To get CRISPR targets in genome assembly ZV9, please click HERE