Expression of long non-coding RNA DLX6-AS1 in lung adenocarcinoma

Juan Li¹, Ping Li¹, Wei Zhao², Rui Yang¹, Shanshan Chen¹, Yong Bai¹, Shaozhi Dun¹,Xiaonan Chen³, Yuwen Du³, Yuanyuan Wang³, Wenqiao Zang³, Guoqiang Zhao³ andGuojun Zhang¹^*

*Corresponding author: Guojun Zhang gjzhangzzu@126.com

Author Affiliations

¹Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China

²Department of Pediatric Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China

³College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China

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Cancer Cell International 2015, 15:48 doi:10.1186/s12935-015-0201-5

The electronic version of this article is the complete one and can be found online at:http://www.cancerci.com/content/15/1/48

Received:	15 December 2014
Accepted:	22 April 2015
Published:	2 May 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Abstract

Background

Lung adenocarcinoma (LAC), the primary histological type of non-small cell lung cancer (NSCLC), has displayed an increasing incidence and mortality worldwide. However, therapeutic approaches were limited. Dysregulation of some lncRNAs has been shown in various types of cancers including LAC. The aim of the present study was to vertify lncRNA DLX6-AS1 expression in LAC.

Methods

Microarray assay revealed expression profile of lncRNAs in LAC. qRT-PCR ( quantitative reverse transcription PCR) was performed to identify lncRNA DLX6-AS1 expression level in 72 paired LAC and adjacent normal lung tissues. qRT-PCR and Western blotting were used to verify that down-regulation lncRNA DLX6-AS1 decreased DLX6 (distal-less homeobox 6) mRNA and protein expression.

Results

Microarray analysis identified up-regulation of 272 lncRNAs and down-regulation of 635 lncRNAs in LAC tissues. The expression level of lncRNA DLX6-AS1 in LAC tissues was significantly higher compared to paired adjacent normal lung tissues (P< 0.05). In addition, its expression level was closed correlated with both histological differentiation (P = 0.004) and TNM stage (P = 0.033). qRT-PCR and Western blotting analysis showed that DLX6 mRNA and protein levels were lower in si-LncRNA group than in the NC (negative control) and Blank groups.

Conclusions

Microarray analysis identified that lncRNA DLX6-AS1 was up-regulated in LAC tissues. High DLX6-AS1 expression levels were significantly associated with both histological differentiation and TNM stage. Down-regulation of lncRNA DLX6-AS1 expression decreased the DLX6 mRNA and protein levels.

Keywords:

lncRNA DLX6-AS1; LAC; Microarray assay; DLX6

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