Clin Biochem. 2014 Oct 11. pii: S0009-9120(14)00707-3. doi: 10.1016/j.clinbiochem.2014.10.001. [Epub ahead of print]
The stability of hexacosanoyl lysophosphatidylcholine in dried-blood spot quality control materials for X-linked adrenoleukodystrophy newborn screening.
Abstract
OBJECTIVES:
Newborn screening for X-linked adrenoleukodystrophy utilizes tandem mass spectrometry to analyze dried-blood spot specimens. Quality control materials (dried-blood spots enriched with hexacosanoyl lysophosphatidylcholine) were prepared and stored at different temperatures for up to 518days to evaluate the stability of this biomarker for X-linked adrenoleukodystrophy.
DESIGN AND METHODS:
Dried-blood spot storage included desiccant (45, 171, and 518days) or omitted desiccant (53days at >90% relative humidity). Specimens were stored for 171 and 518days at -20°C, 4°C, ambient temperature, and 37°C. Each weekday for 45days, a bag of specimens stored at 4°C was warmed to ambient temperature and one specimen was removed for storage at -80°C. Specimens were analyzed by high-performance liquid-chromatography electrospray ionization tandem mass spectrometry and data was plotted as concentration (micromoles per liter) vs. time. Linear regression provided slope and y-intercept values for each storage condition.
RESULTS:
Small slope values (0.01 or less) and y-intercept values close to the enrichment indicated less than 11% loss of hexacosanoyl lysophosphatidylcholine under all storage conditions tested.
CONCLUSIONS:
Quality control materials for X-linked adrenoleukodystrophy are stable for at least 1year when stored with desiccant.
Copyright © 2014. Published by Elsevier Inc.
KEYWORDS:
Dried-blood spot; Lysophosphatidylcholine; Newborn screening; Tandem mass spectrometry; X-linked adrenoleukodystrophy
- PMID:
- 25307302
- [PubMed - as supplied by publisher]
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