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Ahead of Print -Human Influenza A(H7N9) Virus Infection Associated with Poultry Farm, Northeastern China - Volume 20, Number 11—November 2014 - Emerging Infectious Disease journal - CDC

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Ahead of Print -Human Influenza A(H7N9) Virus Infection Associated with Poultry Farm, Northeastern China - Volume 20, Number 11—November 2014 - Emerging Infectious Disease journal - CDC


Volume 20, Number 11—November 2014


Human Influenza A(H7N9) Virus Infection Associated with Poultry Farm, Northeastern China

Ming Fan, Biao Huang, Ao Wang, Liquan Deng, Donglin Wu, Xinrong Lu, Qinglong Zhao, Shuang Xu, Fiona Havers, Yanhui Wang, Jing Wu, Yuan Yin, Bingxin Sun, Jianyi Yao, and Nijuan XiangComments to Author 
Author affiliations: Jilin Provincial Center for Disease Control and Prevention, Jilin, China (M. Fan, B. Huang, A. Wang, L. Deng, D. Wu, X. Lu, Q. Zhao, S. Xu)Changchun Prefectural Center for Disease Control and Prevention, Jilin (Y. Wang, J. Wu, Y. Yin, B. Sun)Chinese Center for Disease Control and Prevention, Beijing, China (J. Yao, N. Xiang)Centers for Disease Control and Prevention, Atlanta, Georgia, USA (F. Havers)


We report on a case of human infection with influenza A(H7N9) virus in Jilin Province in northeastern China. This case was associated with a poultry farm rather than a live bird market, which may point to a new focus for public health surveillance and interventions in this evolving outbreak.
Thumbnail of Provinces in China in which human cases of infection with influenza A(H7N9) virus have been confirmed (gray shading). Jilin Province (dark shading), where the case described in this article occurred, shares borders with the Russian Federation and the Democratic People’s Republic of Korea (North Korea).
Figure 1. Provinces in China in which human cases of infection with influenza A(H7N9) virus have been confirmed (gray shading)Jilin Province (dark shading), where the case described in this article occurred, shares borders...
Since it was first reported in spring 2013 (1), influenza A(H7N9) virus has caused 436 confirmed human infections, resulting in 167 deaths as of July 8, 2014 (Chinese Center for Disease Control and Prevention [China CDC], unpub. data). Most cases have occurred in eastern and southern China and have been associated with exposure to poultry at live poultry markets (LPMs). We report a case of human H7N9 virus infection in Jilin Province in northeastern China, an area not contiguous with provinces in which human cases have been previously reported (Figure 1). Furthermore, this case was associated with a small-scale poultry farm, not an LPM.

The Case

On February 15, 2014, a 50-year-old man, owner of a small farm in Changchun, Jilin Province, experienced an isolated fever (axillary temperature 38.3°C). He had no history of underlying medical conditions. The man sought medical care that day at Jilin University Third Hospital and returned to the hospital on February 16, 17, and 19 with ongoing fever. Radiographic imaging showed evidence of pneumonia; he received intravenous administration of azithromycin and xiyanping, a traditional Chinese medicine. On February 19, the man sought care at Jilin University First Hospital, where throat swab samples were taken. The same day, testing of the samples at the local and provincial Centers for Disease Control and Prevention yielded positive results for influenza A(H7N9) virus by real-time reverse transcription PCR (RT-PCR); China CDC confirmed results the next day. Treatment with oseltamivir (150 mg 2×/d) and methylprednisolone (80 mg 2×/d) was initiated, and oxygen was administered by nasal cannula. The man recovered and was discharged from the hospital on March 7.
The man denied exposure to poultry other than on his farm, including to poultry on other farms or in LPMs. He reported no contact with persons who had similar symptoms before onset of his illness. As part of the case investigation, 68 close contacts of the case-patient were monitored for 7 days; 1 contact had influenza-like illness. Throat swab specimens were collected from this person on days 2 and 3 after symptom onset and tested for influenza A(H7N9) by using real-time RT-PCR; results were negative.
The virus from the case-patient’s specimens was isolated in egg culture and designated A/Jilin/10117/2014 (H7N9) (full sequence available from GISAID, accession no. EPI_ISL_161665). The isolate’s 8 genes were similar to those of the virus A/Anhui/ 02/2013 (H7N9) (GISAID accession no. EPI_ISL_141190); nucleotide/amino acid homology was 99.5%/99.6% for hemagglutinin, 99.2%/98.9% for neuraminidase, 99.3%/99.7% for polybasic 1, 96.4%/98.9% for polybasic 2, 97.9%/99.6% for polymerase acidic, 99.7%/99.6% for nucleoprotein, 97.7%/100.0% for matrix protein, and 99.5%/99.1% for nonstructural 

Mr Fan is a chief physician in the Jilin Provincial Center for Disease Prevention and Control, Jilin, China. His research interests focus on epidemiology and infectious disease prevention and control.


We thank Sue Trock for reviewing this manuscript; Jian Zhao for map production; and Lei Yang, Dayan Wang, and Yuelong Shu for the virus sequencing and analysis of virus data. We also thank the colleagues involved in the field investigation, specimen collection and laboratory testing, logistical administration, and support in the response from China CDC, Jilin Provincial CDC, Changchun Prefectural CDC, Siping Prefectural CDC, Jilin Prefectural CDC, Liaoyuan Prefectural CDC, Jingyue, Luyuan, Chaoyang, and Changchun Economic & Technical Development Zone county CDCs in Changchun city, Gongzhuling county CDC in Siping City, and Dongfeng county CDC in Liaoyuan City, as well as the first cohort of the Jilin Field Epidemiology Training Program.
This work was supported by the China–US Collaborative Program on Emerging and Re-emerging Infectious Diseases and a grant from National Ministry of Science and Technology Emergency Research Project on human infection with avian influenza H7N9 virus (Epidemiology Research Project) (KJYJ-2013-01-02).


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DOI: 10.3201/eid2011.140608

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