domingo, 3 de agosto de 2014

Reference panel of cloned HIV-2 plasmid DNA for... [J Clin Virol. 2014] - PubMed - NCBI

Reference panel of cloned HIV-2 plasmid DNA for... [J Clin Virol. 2014] - PubMed - NCBI

 2014 Jul 7. pii: S1386-6532(14)00261-3. doi: 10.1016/j.jcv.2014.06.028. [Epub ahead of print]

Reference panel of cloned HIV-2 plasmid DNA for nucleic acid assay development, evaluation, and quality monitoring.



Currently, no FDA-approved HIV-2 nucleic acid assay is commercially available in the United States, although several laboratories have developed in-house assays to confirm HIV-2 infections. A major limitation in the development of novel HIV-2 diagnostic assays is the lack of reference materials that can be used to evaluate, optimize, and monitor assay performance.


Eleven viral stocks of HIV-2 isolates from various West African countries, including the Ivory Coast, Senegal, and Guinea-Bissau, were used to clone the entire LTR and pol regions from each virus.


We successfully cloned, sequenced, and group classified 22 HIV-2 DNA plasmids including 11 full length LTR (∼849bp) and 11 pol (∼2995bp) sequences. There were eight HIV-2 group A and three group B in both the LTR and pol regions.


This reference panel provides a robust, quantifiable, renewable, and non-infectious set of reagents that can be used for the development and evaluation of new HIV-2 molecular diagnostic assays and quality assurance and quality control reagents for use in the clinical laboratories.
Published by Elsevier B.V.


Clone; HIV-2; HIV-2 group; NAT; Plasmid; Real-time PCR

[PubMed - as supplied by publisher]

No hay comentarios:

Publicar un comentario