domingo, 31 de agosto de 2014

Multilocus typing of Cryptosporidium spp. an... [Int J Parasitol. 2014] - PubMed - NCBI

Multilocus typing of Cryptosporidium spp. an... [Int J Parasitol. 2014] - PubMed - NCBI



 2014 Aug 18. pii: S0020-7519(14)00189-1. doi: 10.1016/j.ijpara.2014.07.006. [Epub ahead of print]

Multilocus typing of Cryptosporidium spp. and Giardia duodenalis from non-human primates in China.

Karim MR1Zhang S1Jian F1Li J1Zhou C1Zhang L2Sun M3Yang G4Zou F5Dong H1Li J6Rume FI7Qi M1Wang R1Ning C1Xiao L8.

Abstract

Non-human primates (NHPs) are commonly infected with Cryptosporidium spp. and Giardia duodenalis. However, molecular characterisation of these pathogens from NHPs remains scarce. In this study, 2,660 specimens from 26 NHP species in China were examined and characterised by PCR amplification of 18S rRNA, 70kDa heat shock protein (hsp70) and 60kDa glycoprotein (gp60) gene loci for Cryptosporidium; and 1,386 of the specimens by ssrRNA, triosephosphate isomerase (tpi) and glutamate dehydrogenase (gdh) gene loci for Giardia. Cryptosporidium was detected in 0.7% (19/2660) specimens of four NHP species including rhesus macaques (0.7%), cynomolgus monkeys (1.0%), slow lorises (10.0%) and Francois' leaf monkeys (6.7%), belonging to Cryptosporidium hominis (14/19) and Cryptosporidium muris (5/19). Two C. hominis gp60 subtypes, IbA12G3 and IiA17 were observed. Based on the tpi locus, G. duodenalis was identified in 2.2% (30/1,386) of specimens including 2.1% in rhesus macaques, 33.3% in Japanese macaques, 16.7% in Assam macaques, 0.7% in white-headed langurs, 1.6% in cynomolgus monkeys and 16.7% in olive baboons. Sequence analysis of the three targets indicated that all of the Giardia-positive specimens belonged to the zoonotic assemblage B. Highest sequence polymorphism was observed at the tpi locus, including 11 subtypes: three known and eight new ones. Phylogenetic analysis of the subtypes showed that most of them were close to the so-called subtype BIV. Intragenotypic variations at the gdh locus revealed six types of sequences (three known and three new), all of which belonged to so-called subtype BIV. Three specimens had co-infection with C. hominis (IbA12G3) and G. duodenalis (BIV). The presence of zoonotic genotypes and subtypes of Cryptosporidium spp. and G. duodenalis in NHPs suggests that these animals can potentially contribute to the transmission of human cryptosporidiosis and giardiasis.
Copyright © 2014. Published by Elsevier Ltd.

KEYWORDS:

China; Cryptosporidium; Giardia; Multilocus typing; Non-human primates

PMID:
 
25148945
 
[PubMed - as supplied by publisher]

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