domingo, 5 de febrero de 2012

Rapid Diagnosis of Pandemic (H1N1) 2009 in Cuba - Vol. 18 No. 2 - February 2012 - Emerging Infectious Disease journal - CDC

Rapid Diagnosis of Pandemic (H1N1) 2009 in Cuba - Vol. 18 No. 2 - February 2012 - Emerging Infectious Disease journal - CDC

Volume 18, Number 2—February 2012


Rapid Diagnosis of Pandemic (H1N1) 2009 in Cuba

Suggested citation for this article
To the Editor: During 2005–2008, the Cuban National Influenza Center (NIC) at the Pedro Kourí Institute in Havana, Cuba, implemented a protocol for influenza surveillance proposed by the Pan American Health Organization and the US Centers for Disease Control and Prevention (1). One of the most essential features of this protocol was strengthening laboratory capacity for surveillance of seasonal influenza and timely detection of a new influenza virus with pandemic potential.
On April 26, 2009, in response to an outbreak of pandemic (H1N1) 2009 in Mexico, NIC proposed an algorithm with 2 phases for processing specimens. The purpose of the algorithm was to identify the novel virus, effectively monitor its circulation in Cuba, and make these data available to the national health authorities and the World Health Organization (WHO) (2).
The first phase of the algorithm was used during April–September 2009. Following the recommendations of WHO (3), this phase included fluorescent antigen tests, nucleic acid extraction by using QIAamp Viral RNA and QIAamp Viral DNA Kits (QIAGEN, Hilden, Germany), and 3 reverse transcription PCR (RT-PCR) assays for typing and subtyping of influenza A viruses. RT-PCRs were used for differential diagnoses, which included 15 other respiratory viruses (47).
At the same time, on the basis of pandemic (H1N1) 2009 virus sequences published on the Global Initiative on Sharing Avian Influenza Data website ( Web Site Icon), we designed a primer set specific for the hemagglutinin gene and we developed an in-house, conventional RT-PCR was designed to enable virus-specific identification. Confirmation was performed by subsequent sequencing of hemagglutinion, nucleoprotein, or neuraminidase genes by using the BigDye Terminator Cycle Sequencing Quick Start Kit (Beckman Coulter Inc., Krefeld, Germany).
Several sequences obtained were submitted to GenBank under accession nos. HM159409–159418 and HM176606–HM17639) (8,9). BLAST ( Web Site Icon) search analysis on sequences obtained from the first cases identified indicated the highest alignment score (>98% identity) with pandemic (H1N1) 2009 virus strains.
The second phase of the algorithm was used during September 2009–August 2010. This phase included automated nucleic acid extraction (QIAcube; QIAGEN) and real-time RT-PCR kits (Pan American Health Organization, Washington, DC, USA, and Centers for Disease Control and Prevention, Atlanta, GA, USA) for detection and characterization of pandemic (H1N1) 2009 virus (10).
During January 3–July 31, 2009, a total of 2,156 specimens were submitted to the NIC for influenza surveillance. During January 2009–August 10, 2010, a total of 14,692 clinical samples were processed. In the next 6 months, the number of specimens submitted to NIC doubled. More specimens (7,978) were submitted during January 1–August 10, 2010, than during all of 2009.
Most (5,601) clinical specimens processed were from patients with influenza-like illness. The highest percentage (45.9%) of influenza-positive samples was detected in specimens from these patients, followed by specimens from patients during outbreaks (18.0%).
Figure A1
Thumbnail of Influenza test results from weekly national summary, Havana, Cuba, epidemiologic week 1, 2009–epidemiologic week 34, 2010. Figure A1. Influenza test results from weekly national summary, Havana, Cuba, epidemiologic week 1, 2009–epidemiologic week 34, 2010.
Pandemic (H1N1) 2009 virus infection peaked during epidemiologic weeks 39–41, 2009 (Figure A1) and co-circulated with influenza A virus (H3N2). This period coincided with the start of school. A total of 285 and 211 specimens were positive for pandemic (H1N1) 2009 virus and influenza A virus (H3N2), respectively. During April 2010, a second peak of pandemic (H1N1) 2009 was detected, and 303 cases were confirmed during epidemiologic weeks 13–16 (Figure A1).
By the time WHO declared the end of the pandemic on August 10, 2010, public health authorities recognized 1,805 cases of pandemic (H1N1) 2009 in Cuba. On the basis of laboratory results, during April 2009–2010, two peaks of pandemic (H1N1) 2009 were observed in Cuba.
During August 2010, Cuba experienced active transmission of seasonal influenza A (H3N2) virus, which displaced pandemic (H1N1) 2009 virus as the predominant virus. Similarly, seasonal influenza virus (H1N1) was displaced by pandemic (H1N1) 2009 virus. The last case of seasonal influenza (H1N1) detected in Cuba was in June 2009.
In this context, we believe that implementing national diagnostic algorithm enabled timely identification of the novel virus and effective monitoring of its circulation, even before international diagnostic protocols and reagents were available in Cuba. This study shows the need for nucleic acid amplification tools in laboratory diagnosis and surveillance of influenza viruses. As we prepare for future influenza pandemics, new and appropriate diagnostic methods and periodic assessment of influenza surveillance methods are needed as new information becomes available.
Belsy AcostaComments to Author , Alexander Piñón, Odalys Valdés, Clara Savón, Amely Arencibía, Elías Guilarte, Gonzalez Grehete, Suset Oropesa, Gonzalez Guelsys, Bárbara Hernández, Angel Goyenechea, Mayra Muné, Vivian Kouri, María G. Guzmán, and Alina Llop
Author affiliations: Instituto Pedro Kourí, Havana, Cuba


We thank the physicians and laboratory personnel for providing samples and clinical data; the technicians and researchers of the Virology Department and Microbiology Division of the Pedro Kourí Institute, Havana, Cuba, for collaborating in the diagnosis of pandemic influenza; Thais dos Santos and Mauricio Cerpa for revising the manuscript; and the US Centers for Disease Control and Prevention, the Chinese Centers for Disease Control (Beijing, China), and the Carlos III Institute (Madrid, Spain) for providing test kits and positive controls.
This study was supported in part by the Cuban National Program of Surveillance and Control for Acute Respiratory Infection of the Ministry of Health and the Pan American Health Organization.


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Suggested citation for this article: Acosta B, Piñón A, Valdés O, Savón C, Arencibía A, Guilarte E, et al. Rapid diagnosis of pandemic (H1N1) 2009 in Cuba [letter]. Emerg Infect Dis [serial on the Internet]. 2012 Feb [date cited]. Web Site Icon
DOI: 10.3201/eid1802.110547

1 comentario:

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