Molecular and cellular characterizations of human cherubism: disease aggressiveness depends on osteoclast differentiation

Natacha KadlubEmail author View ORCID ID profile,
Quentin Sessiecq,
Marion Mandavit,
Aurore Coulomb L’Hermine,
Cecile Badoual,
Louise Galmiche,
Ariane Berdal,
Vianney Descroix†,
Arnaud Picard† and
Amélie E. Coudert†

†Contributed equally

Orphanet Journal of Rare Diseases201813:166

https://doi.org/10.1186/s13023-018-0907-2

Received: 19 December 2017
Accepted: 12 September 2018
Published: 20 September 2018

Abstract

Background

Cherubism is a rare autosomal dominant disorder of the jaws caused by mutation of the SH3BP2 gene. The bone is replaced by a fibrous granuloma containing multinucleated giant cells. Cells of the cherubism granuloma have never been systematically analyzed. Hence, the aim of this study was to characterize the cells in human cherubism granulomas, to determine the osteoclastic characteristics of the multinucleated giant cells and to investigate the potential role of TNF-α in human cherubism.

Results

Seven granulomas were analyzed in pathology, molecular biology and immunohistochemistry. Granulomas were composed mainly of macrophages or osteoclasts within a fibroblastic tissue, with few lymphoid cells. Myeloid differentiation and nuclear NFATc1 localization were both associated with disease aggressiveness. OPG and RANKL immunohistochemical expression was unexpected in our specimens. Five granuloma cells were cultured in standard and osteoclastogenic media. In culture, cherubism cells were able to differentiate into active osteoclasts, in both osteoclastogenic and standard media. IL-6 was the major cytokine present in the culture supernatants.

Conclusion

Multinucleated giant cells from cherubism granulomas are CD68 positive cells, which differentiate into macrophages in non-aggressive cherubism and into osteoclasts in aggressive cherubism, stimulated by the NFATc1 pathway. This latter differentiation appears to involve a disturbed RANK-L/RANK/OPG pathway and be less TNF-α dependent than the cherubism mouse model.