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Ahead of Print -Novel Arenavirus Isolates from Namaqua Rock Mice, Namibia, Southern Africa - Volume 21, Number 7—July 2015 - Emerging Infectious Disease journal - CDC

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Ahead of Print -Novel Arenavirus Isolates from Namaqua Rock Mice, Namibia, Southern Africa - Volume 21, Number 7—July 2015 - Emerging Infectious Disease journal - CDC



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Volume 21, Number 7—July 2015

Dispatch

Novel Arenavirus Isolates from Namaqua Rock Mice, Namibia, Southern Africa

Peter T. WitkowskiComments to Author , René Kallies, Julia Hoveka, Brita Auste, Ndapewa L. Ithete, Katarína Šoltys, Tomáš Szemes, Christian Drosten, Wolfgang Preiser, Boris Klempa, John K.E. Mfune, and Detlev H. Kruger
Author affiliations: Charité Medical School, Berlin, Germany (P.T. Witkowski, B. Auste, B. Klempa, D.H. Kruger);Helmholtz Centre for Environmental Research–UFZ, Leipzig, Germany (R. Kallies)University of Bonn Medical Center, Bonn, Germany (R. Kallies, C. Drosten)University of Namibia, Windhoek, Namibia (J. Hoveka, J.K.E. Mfune)Stellenbosch University and National Health Laboratory Services Tygerberg, Cape Town, South Africa (N.L. Ithete, W. Preiser)Comenius University, Bratislava, Slovakia (K. Šoltys, T. Szemes)Slovak Academy of Sciences, Bratislava (B. Klempa)

Abstract

Arenaviruses are feared as agents that cause viral hemorrhagic fevers. We report the identification, isolation, and genetic characterization of 2 novel arenaviruses from Namaqua rock mice in Namibia. These findings extend knowledge of the distribution and diversity of arenaviruses in Africa.
Arenaviruses are known to cause severe hemorrhagic fevers across the globe with case fatality rates up to 30% (1). The viruses possess a bisegmented, single-stranded RNA genome with ambisense coding strategy consisting of a small segment coding for the nucleoprotein and glycoprotein and a large (L) segment coding for the RNA-dependent RNA polymerase and matrix protein.
In Africa, Lassa virus (LASV) and Lujo virus are the only known members of the family Arenaviridae that cause human disease (2,3); however, evidence for lymphocytic choriomeningitis virus, another Arenaviridae sp., was recently reported in Gabon (4). Several other arenaviruses of unknown pathogenic potential have also been found in Africa: Gbagroube, Kodoko, and Menekre viruses from western Africa (5,6); Ippy (IPPYV) and Mobala viruses from central Africa; Mopeia, Morogoro, Luna, and Lunk viruses from eastern Africa; and Merino Walk virus (MWV) from southern Africa (7,8). All of these viruses are carried by rodents of the family Muridae.
Until now, no molecular detection of arenaviruses has been reported from Namibia. A study in 1991 described a low seroprevalence (0.8%) for LASV antibodies in humans in northern Namibia (9). Because of lack of data about arenavirus occurrence and effects in southwestern Africa, we conducted a study of small mammals from Namibia to detect infection with arenaviruses.

Dr. Witkowski is a postdoctoral researcher at the Institute of Virology of the Charité Medical School in Berlin, Germany. His research interests are viral emerging infectious diseases on the African continent and their clinical impact and evolution.

Acknowledgments

We thank C. Chimimba for advice in small mammal systematics, P. Chimwamurombe for advice during preliminary screening for arenaviruses, and C. Priemer for technical support.
Trapping in Namibia was carried out under research permit nos. 1572/2011, 1666/2012 and 1794/2013, granted by Namibia’s Ministry of Environment and Tourism.
This study was supported by Deutsche Forschungsgemeinschaft (grant KR1293/13-1) and by the Slovak Research and Development Agency under the contract no. DO7RP-0008-09.

References

  1. Günther SLenz OLassa virus. Crit Rev Clin Lab Sci2004;41:33990DOIPubMed
  2. Briese TPaweska JTMcMullan LKHutchison SKStreet CPalacios GGenetic detection and characterization of Lujo virus, a new hemorrhagic fever-associated arenavirus from southern Africa. PLoS Pathog2009;5:e1000455DOIPubMed
  3. Frame JDBaldwin JMGocke DJTroupt AMLassa fever, a new virus disease of man from West Africa. Am J Trop Med Hyg.1970;19:6706.PubMed
  4. N’Dilimabaka NBerthet NRougeron VMangombi JBDurand PMaganga GDEvidence of lymphocytic choriomeningitis virus (LCMV) in domestic mice in Gabon: risk of emergence of LCMV encephalitis in Central Africa. J Virol2015;89:145660DOIPubMed
  5. Lecompte EterMeulen JEmonet SDaffis SCharrel RNGenetic identification of Kodoko virus, a novel arenavirus of the African pigmy mouse (Mus(Nannomysminutoides) in West Africa. Virology2007;364:17883DOIPubMed
  6. Coulibaly-N’Golo DAllali BKouassi SKFichet-Calvet EBecker-Ziaja BRieger TNovel arenavirus sequences in Hylomyscus sp. and Mus(Nannomyssetulosus from Côte d’Ivoire: implications for evolution of arenaviruses in Africa. PLoS ONE2011;6:e20893DOIPubMed
  7. Ishii AThomas YMoonga LNakamura IOhnuma AHang’ombe BNovel arenavirus, Zambia. Emerg Infect Dis2011;17:19214DOIPubMed
  8. Zapata JCSalvato MSArenavirus variations due to host-specific adaptation. Viruses2013;5:24178DOIPubMed
  9. Joubert JJvan der Merve CALourens JHLecatsas GSiegrühn CSerological markers of hepatitis B virus and certain other viruses in the population of eastern Caprivi, Namibia. Trans R Soc Trop Med Hyg1991;85:1013DOIPubMed
  10. Vieth SDrosten CLenz OVincent MOmilabu SHass MRT-PCR assay for detection of Lassa virus and related Old World arenaviruses targeting the L gene. Trans R Soc Trop Med Hyg2007;101:125364DOIPubMed
  11. Palacios GQuan PJabado OJConlan SHirschberg DLLiu YPanmicrobial oligonucleotide array for diagnosis of infectious diseases. Emerg Infect Dis2007;13:7381DOIPubMed
  12. Kearse MMoir RWilson AStones-Havas SCheung MSturrock SGeneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics2012;28:16479DOIPubMed
  13. Tamura KStecher GPeterson DFilipski AKumar SMEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol.2013;30:27259DOIPubMed
  14. Index of viruses—arenaviridae. In: Büchen-Osmond C, editor. The Universal Virus Database, version 4. New York: Columbia University, 2006 [cited 2014 Aug 11]. www.ncbi.nlm.nih.gov/ICTVdb/Ictv/fs_index.htm
  15. Coetzee NGriffin MTaylor PJAethomys namaquensis. In: The International Union for Conservation of Nature Red List of Threatened Species. Version 2014.1. 2014 [cited 2014 Aug 11]. www.iucnredlist.org

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Suggested citation for this article: Witkowski PT, Kallies R, Hoveka J, Auste B, Ithete NL, Šoltys T, et al. Novel arenavirus isolates from Namaqua rock mice, Namibia, Southern Africa. Emerg Infect Dis. 2015 Jul [date cited]. http://dx.doi.org/10.3201/eid2107.141341
DOI: 10.3201/eid2107.141341

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