Infect Genet Evol. 2013 Nov 21;21C:214-219. doi: 10.1016/j.meegid.2013.11.004. [Epub ahead of print]
Genetic analysis of G12P rotaviruses detected in the largest U.S. G12 genotype outbreak on record.
Mijatovic-Rustempasic S1, Teel EN1, Kerin TK1, Hull JJ1, Roy S1, Weinberg GA2, Payne DC1, Parashar UD1, Gentsch JR1, Bowen MD3.
In 2006-07, 77 cases of gastroenteritis in Rochester, NY, USA were associated with rotavirus genotype G12P. Sequence analysis identified a high degree of genetic relatedness among the VP7 and VP4 genes of the Rochester G12P strains and between these strains and currently circulating human G12P strains. Out of 77 samples, two and seven unique nucleotide sequences were identified for VP7 and VP4 genes, respectively. Rochester strain VP7 genes were found to occupy the G12-III lineage and VP4 genes clustered within the P-3 lineage. Six strains contained non-synonymous nucleotide substitutions that produced amino acid changes at 6 sites in the VP8∗ region of the VP4 gene. Two sites (amino acids 242 and 246) were located in or near a described trypsin cleavage site. Selection analyses identified one positively selected VP7 site (107) and strong purifying selection at 58 sites within the VP7 gene as well as 2 of the 6 variant sites (79 and 218) in VP4.
Published by Elsevier B.V.
Rotavirus, USA, VP4 protein, VP7 protein, VP8(∗), Vaccine
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