CHOP regulates the p53–MDM2 axis and is required for neuronal survival after seizures
- Tobias Engel1,2,
- Amaya Sanz-Rodgriguez1,2,
- Eva M. Jimenez-Mateos1,2,
- Caoimhin G. Concannon1,2,
- Alba Jimenez-Pacheco1,2,
- Catherine Moran1,
- Guillaume Mesuret1,2,
- Emilie Petit3,
- Norman Delanty4,
- Michael A. Farrell4,
- Donncha F. O’Brien4,
- Jochen H. M. Prehn1,2,
- Jose J. Lucas5,6 and
- David C. Henshall1,2
+ Author Affiliations
1 Department of Physiology and Medical Physics, Royal College of Surgeons in Ireland, Dublin, 2, Ireland
2 Centre for the Study of Neurological Disorders, Royal College of Surgeons in Ireland, Dublin, 2, Ireland
3 Molecular and Cellular Therapeutics, Royal College of Surgeons in Ireland, Dublin, 2, Ireland
4 Beaumont Hospital, Dublin, 9, Ireland
5 Center for Molecular Biology ‘Severo Ochoa’ (CBMSO), CSIC/UAM, Madrid, Spain
6 Networking Research Center on Neurodegenerative Diseases (CIBERNED), Instituto de Salud Carlos III, Madrid, Spain
- Correspondence to: David C. Henshall, Department of Physiology and Medical Physics, Royal College of Surgeons in Ireland, 123 St. Stephen’s Green, Dublin 2, Ireland E-mail: email@example.com
- Received July 23, 2012.
- Revision received October 5, 2012.
- Accepted November 4, 2012.
Hippocampal sclerosis is a frequent pathological finding in patients with temporal lobe epilepsy and can be caused by prolonged single or repeated brief seizures. Both DNA damage and endoplasmic reticulum stress have been implicated as underlying molecular mechanisms in seizure-induced brain injury. The CCAAT/enhancer-binding protein homologous protein (CHOP) is a transcriptional regulator induced downstream of DNA damage and endoplasmic reticulum stress, which can promote or inhibit apoptosis according to context. Recent work has proposed inhibition of CHOP as a suitable neuroprotective strategy. Here, we show that transcript and protein levels of CHOP increase in surviving subfields of the hippocampus after prolonged seizures (status epilepticus) in mouse models. CHOP was also elevated in the hippocampus from epileptic mice and patients with pharmacoresistant epilepsy. The hippocampus of CHOP-deficient mice was much more vulnerable to damage in mouse models of status epilepticus. Moreover, compared with wild-type animals, CHOP-deficient mice subject to status epilepticus developed more spontaneous seizures, displayed protracted hippocampal neurodegeneration and a deficit in a hippocampus-dependent object–place recognition task. The absence of CHOP was associated with a supra-maximal induction of p53 after status epilepticus, and inhibition of p53 abolished the cell death-promoting consequences of CHOP deficiency. The protective effect of CHOP could be partly explained by activating transcription of murine double minute 2 that targets p53 for degradation. These data demonstrate that CHOP is required for neuronal survival after seizures and caution against inhibition of CHOP as a neuroprotective strategy where excitotoxicity is an underlying pathomechanism.
- cornu ammonis
- CCAAT/enhancer-binding protein homologous protein
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