NDM-1–producing Klebsiella pneumoniae, Croatia

Suggested citation for this article

To the Editor: The novel metallo-β-lactamase named New Delhi metallo-β-lactamase (NDM-1) was identified from Klebsiella pneumoniae and Escherichia coli isolates in Sweden from a patient previously hospitalized in India (1). NDM-1 is spreading rapidly worldwide to nonclonally related isolates, many of which are directly or indirectly tracked to the Indian subcontinent (2). A carbapenem-resistant K. pneumoniae strain, KLZA, was isolated in May 2009 from the culture of a blood sample from of a 40-year-old man on the day after his admission to a surgical intensive care unit of the Clinical Hospital Center in Zagreb, Croatia. The patient had been transferred after 5 days of hospitalization in Bosnia and Herzegovina following a car accident. The clinical history mentioned antimicrobial drug treatment that did not include carbapenems (gentamicin, metronidazole, and ceftriaxone) and no link to the Indian subcontinent. Antimicrobial drug susceptibility testing was performed by Vitek2 (bioMérieux, Marcy-l’Etoile, France) and broth microdilution and interpreted according to the latest documents from the European Committee on Antimicrobial Susceptibility Testing (www.eucast.org/clinical_breakpoints/, version 1.1).

The strain proved resistant to imipenem and meropenem, to all broad-spectrum cephalosporins, and to aminoglycosides and susceptible to ciprofloxacin and tigecycline (Table). We checked for bla_VIM, bla_IMP, bla_SPM, bla_GIM, bla_SIM, and bla_NDM resistance genes by using PCR. A PCR product was obtained only with the NDM primers, after being purified (QIAquick PCR Purification Kit, QIAGEN, Hilden, Germany), its sequence showed 100% identity with bla_NDM-1.

Strain genotyping was performed by multilocus sequence typing to determine the sequence type (ST) of the isolate and to establish a comparison with previously reported NDM-1–producing isolates. Allelic numbers were obtained on the basis of sequences of 7 housekeeping genes at www.pasteur.fr/recherche/genopole/PF8/mlst/Kpneumoniae.html. Multilocus sequence typing identified K. pneumoniae KLZA as an ST25 strain, which significantly differs from the ST14 type found in the index NDM-1–producing strain and from other isolates originating from India (1) and then in other countries. ST25 K. pneumoniae was also found in K. pneumoniae isolates in Geneva (3). Other K. pneumoniae STs harboring NDM-1 were ST15, ST16, and ST147 (4–7).

Resistance was transferred by conjugation to E. coli J53, with selection based on growth on agar in the presence of ceftazidime (10 mg/L) and azide (100 mg/L). The conjugant T1 showed resistance to β-lactams, including all carbapenems, as well as decreased susceptibility to ciprofloxacin.

The KLZA strain and its transconjugant harbored other determinant of resistance, namely bla_CTX-M-15, bla_CMY-16, and qnrA6. Plasmid incompatibility groups, determined by a PCR-based replicon typing method, belonged to the incA/C replicon type.

This report of an NDM-1–producing K. pneumoniae in Croatia adds to those of other cases in patients from patients hospitalized in the Balkan area. The patient in this report had no apparent link to the Indian subcontinent.

In a survey conducted by the European Centre for Disease Prevention and Control to gather information about the spread of NDM-1–producing Enterobacteriaceae in Europe and reporting cases from 13 countries during 2008–2010, five of the 55 persons with known travel histories had traveled to the Balkan region during the month before diagnosis of their infection: 2 to Kosovo and 1 each to Serbia, Montenegro, and Bosnia and Herzegovina. All had received hospital care in Balkan countries because of an illness or accident that occurred during the journey (7). Two of the latter cases (4,8) and a case from Germany (9) were subsequently published. No patient had any apparent link to the Indian subcontinent.

Although the way NDM-1 isolates might have been imported to western Europe not only from the Indian subcontinent but also from Balkan countries (10) has been highlighted, awareness of western Europe as a possible area of endemicity remains limited. The aforementioned report from Germany, although recognizing that the patient had been repatriated after hospitalization in Serbia, declared “no evidence about contact with people from regions where NDM-enterobacteria are endemic” (9). This limited awareness shows the threat of neglecting to screen patients who are transferred from countries thought not to be at risk for NDM-1. Furthermore, it means that specimen are not sent to the local reference laboratories and recognized as positive for NDM-1, thus permitting wide dissemination of NDM-1–producing enterobacteria in the community (4). The accumulating evidence of NDM-1 from the Balkan area could suggest a possible multifocal spread of this enzyme, with the Balkans as a possible second area of endemicity, in addition to the Indian subcontinent, and prompts for widespread epidemiologic surveillance.

Annarita Mazzariol

, Zrinka Bošnjak, Piero Ballarini, Ana Budimir, Branka Bedenić, Smilja Kalenić, and Giuseppe Cornaglia

Author affiliations: University of Verona, Verona, Italy (A. Mazzariol, P. Ballarini, G. Cornaglia); University of Zagreb, Zagreb, Croatia (Z. Bošnjak, A. Budimir, B. Bedenić, S. Kalenić)

References

Yong D, Toleman MA, Giske CG, Cho HS, Sundman K, Lee K, Characterization of a new metallo-β-lactamase gene, bla_NDM-1, and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. Antimicrob Agents Chemother. 2009;53:5046–54. DOIPubMed
Rolain JM, Parola P, Cornaglia G. New Delhi metallo-beta-lactamase (NDM-1): towards a new pandemia? Clin Microbiol Infect. 2010;16:1699–701. DOIPubMed
Poirel L, Schrenzel J, Cherkaoui A, Bernabeu S, Renzi G, Nordmann P. Molecular analysis of NDM-1–producing enterobacterial isolates from Geneva, Switzerland. J Antimicrob Chemother. 2011;66:1730–3. DOIPubMed
Bogaerts P, Bouchahrouf W, de Castro RR, Deplano A, Berhin C, Piérard D, et al. Emergence of NDM-1–producing Enterobacteria in Belgium. Antimicrob Agents Chemother. 2011;55:3036–8. DOIPubMed
Mulvey MR, Grant JM, Plewes K, Roscoe D, Boyd DA. New Delhi metallo-β-lactamase in Klebsiella pneumoniae and Escherichia coli, Canada. Emerg Infect Dis. 2011;17:103–6. DOIPubMed
Sidjabat H, Nimmo GR, Walsh TR, Binotto E, Htin A, Hayashi Y. Carbapenem resistance in Klebsiella pneumoniae due to the New Delhi metallo-β-lactamase. Clin Infect Dis. 2011;52:481–4. DOIPubMed
Struelens MJ, Monnet DL, Magiorakos AP, O’Connor FS, Giesecke J. European NDM-1 Survey Participants. New Delhi metallo-beta-lactamase 1–producing Enterobacteriaceae: emergence and response in Europe. Euro Surveill. 2010;15:pii:19716. PubMed
Hammerum AM, Toleman MA, Hanse F, Kristensen B, Lester CH, Walsh TR, Global spread of New Delhi metallo-β-lactamase 1. Lancet Infect Dis. 2010;10:829–30. DOIPubMed
Göttig S, Pfeifer Y, Wichelhas TA, Zacharowski K, Bingold T, Averhoff B, Global spread of New Delhi metallo-β-lactamase 1. Lancet Infect Dis. 2010;10:828–9. DOIPubMed
Livermore DM, Walsh TR, Toleman M, Woodford N. Balkan NDM-1: escape or transplant? Lancet Infect Dis. 2011;11:164. DOIPubMed

Table

Table. MIC of the KLZA strain of Klebsiella pneumoniae and its transconjugant and recipient

Suggested citation for this article: Mazzariol A, Bošnjak Z, Ballarini P, Budimir A, Bedenić B, Kalenić S, et al. NDM-1–producing Klebsiella pneumoniae, Croatia [letter]. Emerg Infect Dis [serial on the Internet] 2012 Mar [date cited]. http://dx.doi.org/10.3201/eid1803.110389

DOI: 10.3201/eid1803.110389