lunes, 11 de noviembre de 2013

Development of a reverse genetics system to generat... [Virology. 2013] - PubMed - NCBI

Development of a reverse genetics system to generat... [Virology. 2013] - PubMed - NCBI

Virology. 2013 Nov;446(1-2):230-7. doi: 10.1016/j.virol.2013.07.038. Epub 2013 Sep 5.

Development of a reverse genetics system to generate recombinant Marburg virus derived from a bat isolate.


Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.


Recent investigations have shown the Egyptian fruit bat (Rousettus aegyptiacus) to be a natural reservoir for marburgviruses. To better understand the life cycle of these viruses in the natural host, a new reverse genetics system was developed for the reliable rescue of a Marburg virus (MARV) originally isolated directly from a R. aegyptiacus bat (371Bat). To develop this system, the exact terminal sequences were first determined by 5' and 3' RACE, followed by the cloning of viral proteins NP, VP35, VP30 and L into expression plasmids. Novel conditions were then developed to efficiently replicate virus mini-genomes followed by the construction of full-length genomic clones from which recombinant wild type and GFP-containing MARVs were rescued. Surprisingly, when these recombinant MARVs were propagated in primary human macrophages, a dramatic difference was found in their ability to grow and to elicit anti-viral cytokine responses.
Published by Elsevier Inc.


Bat isolate, Cytokines, Filovirus, Full-length, GFP, Macrophages, Marburg virus, Marburgvirus, Minigenome, Recombinant


[PubMed - in process]

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