Identification of Genetic Loci Associated With Helicobacter pylori Serologic Status

Julia Mayerle, MD; Caroline M. den Hoed, MD; Claudia Schurmann, MSc; Lisette Stolk, PhD; Georg Homuth, PhD; Marjolein J. Peters, MD, PhD; Lisette G. Capelle, MD; Kathrin Zimmermann, MD; Fernando Rivadeneira, MD, PhD; Sybille Gruska, PhD; Henry Völzke, MD; Annemarie C. de Vries, PhD; Uwe Völker, PhD; Alexander Teumer, PhD; Joyce B. J. van Meurs, PhD; Ivo Steinmetz, MD; Matthias Nauck, MD; Florian Ernst, PhD; Frank-Ulrich Weiss, PhD; Albert Hofman, MD, PhD; Martin Zenker, MD; Heyo K. Kroemer, PhD; Holger Prokisch, PhD; Andre G. Uitterlinden, MD, PhD; Markus M. Lerch, MD, FRCP; Ernst Kuipers, MD, PhD

JAMA. 2013;309(18):1912-1920. doi:10.1001/jama.2013.4350.

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ABSTRACT

Importance Helicobacter pylori is a major cause of gastritis and gastroduodenal ulcer disease and can cause cancer. H pylori prevalence is as high as 90% in some developing countries but 10% of a given population is never colonized, regardless of exposure. Genetic factors are hypothesized to confer H pylori susceptibility.
Objective To identify genetic loci associated with H pylori seroprevalence in 2 independent population-based cohorts and to determine their putative pathophysiological role by whole-blood RNA gene expression profiling.
Design, Setting, and Participants Two independent genome-wide association studies (GWASs) and a subsequent meta-analysis were conducted for anti-H pylori IgG serology in the Study of Health in Pomerania (SHIP) (recruitment, 1997-2001 [n = 3830]) as well as the Rotterdam Study (RS-I) (recruitment, 1990-1993) and RS-II (recruitment, 2000-2001 [n = 7108]) populations. Whole-blood RNA gene expression profiles were analyzed in RS-III (recruitment, 2006-2008 [n = 762]) and SHIP-TREND (recruitment, 2008-2012 [n = 991]), and fecal H pylori antigen in SHIP-TREND (n = 961).
Main Outcomes and Measures H pylori seroprevalence.
Results Of 10 938 participants, 6160 (56.3%) were seropositive for H pylori. GWASs identified the toll-like receptor (TLR) locus (4p14; top-ranked single-nucleotide polymorphism (SNP), rs10004195; P = 1.4 × 10⁻¹⁸; odds ratio, 0.70 [95% CI, 0.65 to 0.76]) and the FCGR2A locus (1q23.3; top-ranked SNP, rs368433; P = 2.1 × 10⁻⁸; odds ratio, 0.73 [95% CI, 0.65 to 0.81]) as associated with H pylori seroprevalence. Among the 3 TLR genes at 4p14, only TLR1 was differentially expressed per copy number of the minor rs10004195-A allele (β = −0.23 [95% CI, −0.34 to −0.11]; P = 2.1 × 10⁻⁴). Individuals with high fecal H pylori antigen titers (optical density >1) also exhibited the highest 25% of TLR1 expression levels (P = .01 by χ² test). Furthermore, TLR1 exhibited an Asn248Ser substitution in the extracellular domain strongly linked to the rs10004195 SNP.
Conclusions and Relevance GWAS meta-analysis identified an association between TLR1 and H pylori seroprevalence, a finding that requires replication in nonwhite populations. If confirmed, genetic variations in TLR1 may help explain some of the observed variation in individual risk for H pylori infection.