Evaluation of new biomarker genes for differentiating Haemophilus influenzae from Haemophilus haemolyticus.

Theodore MJ, Anderson RD, Wang X, Katz LS, Vuong JT, Bell ME, Juni BA, Lowther SA, Lynfield R, Macneil JR, Mayer LW.

Source

Meningitis and Vaccine Preventable Diseases Branch, Centers for Disease Control and Prevention (CDC), 1600 Clifton Road, MS D11; Atlanta, Georgia 30333.

Abstract

PCR detecting the protein D (hpd) and fuculose kinase (fucK) genes showed high sensitivity and specificity for identifying Haemophilus influenzae and differentiating it from H. haemolyticus. Phylogenetic analysis using the 16S rRNA gene demonstrated two distinct groups for H. influenzae and H. haemolyticus.