Volume 17, Number 11—November 2011
THEME ISSUE
CHOLERA IN HAITI
Dispatch
Multinational Cholera Outbreak after Wedding in the Dominican Republic
Article Contents
Mercedes Laura Jiménez
, Andria Apostolou, Alba Jazmin Palmera Suarez, Luis Meyer, Salvador Hiciano, Anna Newton, Oliver Morgan, Cecilia Then, and Raquel Pimentel
Suggested citation for this article , Andria Apostolou, Alba Jazmin Palmera Suarez, Luis Meyer, Salvador Hiciano, Anna Newton, Oliver Morgan, Cecilia Then, and Raquel PimentelAbstract
We conducted a case–control study of a cholera outbreak after a wedding in the Dominican Republic, January 22, 2011. Ill persons were more likely to report having consumed shrimp on ice (odds ratio 8.50) and ice cubes in beverages (odds ratio 3.62). Travelers to cholera-affected areas should avoid consuming uncooked seafood and untreated water.On January 25, 2011, the Dominican Republic Ministry of Health was notified that 5 Venezuelan nationals had been hospitalized with cholera-like illness. All patients had attended a large wedding reception at a luxury tourist resort in La Romana Province, Dominican Republic, on January 22. At the reception were 216 local workers and 540 guests; ≈90% were residents of Venezuela, and the others were from the Dominican Republic, Mexico, the United States, and Spain. In La Romana Province, no other cases had previously been identified, and during the week of the outbreak, all reported cases were associated with the wedding.
We conducted a case–control study to identify foods and beverages consumed at the wedding banquet that were associated with diarrheal illness. A questionnaire developed by Dominican Republic health authorities, which addressed only food items consumed at the banquet, was sent to national health authorities in the 4 countries where guests originated. They were asked to administer it to wedding attendees and return completed questionnaires to the Dominican Republic for analysis.
Case-patients were identified from routine surveillance reports in the Dominican Republic and the United States. We also actively sought case-patients by asking the venue personnel, event organizer, and other case-patients to inform us about other persons at the banquet from the same country of origin. A patient with a suspected case was defined as a person who had watery diarrhea during January 22–28, 2011, and had consumed food and beverages at the banquet. In addition to meeting suspected-case criteria, a patient with a confirmed case also had laboratory confirmation of infection by culture for Vibrio cholerae O1 or agglutination for serotype Ogawa. Controls were identified by event organizers or other banquet attendees as guests or workers at the banquet who consumed food and beverages but did not have diarrhea during January 22–28, 2011. We intended to recruit at least twice as many controls as case-patients. We calculated odds ratios (ORs) and 95% confidence intervals (CIs) regarding whether consuming particular foods and beverages was associated with being a case-patient (with suspected and confirmed cases) and controls. Food and beverage items potentially associated with illness in a univariable analysis (p<0.10) were included as independent dichotomous predictor variables in a multivariable logistic regression model with case status as the dependent dichotomous variable. We used StataCorp 2005 Release 9 (College Station, TX, USA) for the analysis. A Ministry of Health environmental inspector interviewed the event caterers about purchase, transport, storage, preparation, and service of food and beverages at the banquet.
We identified 42 case-patients (25 with confirmed and 17 with suspected cases) and 62 controls; all agreed to participate in the study. Questionnaires were completed between January 24 and March 3, 2011. Twenty-two (51%) case-patients and 59 (97%) controls were from the Dominican Republic (Table 1). Of case-patients, 24 (57%) were wedding guests, 16 (38%) were workers, and 2 (5%) did not attend the reception but ate leftover food from the banquet. Of 62 controls, only 6 (10%) were guests and 56 (90%) were workers (Table 1). Only data from Venezuelan case-patients who received a diagnosis in the Dominican Republic were available for analysis. We did not specify how case-patients were identified.
Figure. Date and time of onset of cholera cases (N = 40) associated with wedding in La Romana, Dominican Republic, January 2011 (2 case-patients not represented here because time of symptom onset...Median age of case-patients was 42.5 years (range 16–84 years); 33 (79%) were male. All experienced watery diarrhea, accompanied by dehydration (28 patients [67%]), nausea (13 [30%]), vomiting (15 [36%]), or cramps (8 [19%]). Time to illness onset was 10 hours to 6 days (Figure). Thirty-four (81%) case-patients were given antimicrobial drugs, and 22 (52%) were hospitalized; all recovered.
Odds were higher for case-patients than controls for having consumed cooked shrimp on ice (OR 8.5, 95% CI 3.3–21.71), cooked langostinos (squat lobster) (OR 2.9, 95% CI 1.1–7.7), and beverages with ice cubes (OR 3.6, 95% CI 1.4–9.3), but odds were lower for having consumed mixed rice (OR 0.1, 95% CI 0.01–0.9) or other foods (OR 0.13, 95% CI 0.05–1.03) (Table A1). When these variables were included in a multivariable logistic regression, cooked shrimp on ice (OR 10.8, 95% CI 3.3–35.4), ice cubes (OR 4.1, 95% CI 1.3–13.2), and mixed rice (OR 0.04, 95% CI 0.003–0.5) remained significantly associated with case status (Table 2).
Environmental inspection found that the food was prepared by a caterer outside of the resort. Shrimp and lobster came from the Dominican Republic (Higuey in La Altagracia Province), and the langostinos came from Beata Island in Perdenales Province and Anse-A-Pitre in southeastern Haiti. No samples were available for testing. Inspection of the caterer’s kitchen revealed poor food-handling practices, including improper refrigeration, poor hand hygiene, and nonchlorinated water supply. On the night of the wedding reception, food was set out at 7:00pm but not consumed until 10:00 pm . Shrimp and langostinos were served on ice or ice sculptures.
Case-patients were identified from routine surveillance reports in the Dominican Republic and the United States. We also actively sought case-patients by asking the venue personnel, event organizer, and other case-patients to inform us about other persons at the banquet from the same country of origin. A patient with a suspected case was defined as a person who had watery diarrhea during January 22–28, 2011, and had consumed food and beverages at the banquet. In addition to meeting suspected-case criteria, a patient with a confirmed case also had laboratory confirmation of infection by culture for Vibrio cholerae O1 or agglutination for serotype Ogawa. Controls were identified by event organizers or other banquet attendees as guests or workers at the banquet who consumed food and beverages but did not have diarrhea during January 22–28, 2011. We intended to recruit at least twice as many controls as case-patients. We calculated odds ratios (ORs) and 95% confidence intervals (CIs) regarding whether consuming particular foods and beverages was associated with being a case-patient (with suspected and confirmed cases) and controls. Food and beverage items potentially associated with illness in a univariable analysis (p<0.10) were included as independent dichotomous predictor variables in a multivariable logistic regression model with case status as the dependent dichotomous variable. We used StataCorp 2005 Release 9 (College Station, TX, USA) for the analysis. A Ministry of Health environmental inspector interviewed the event caterers about purchase, transport, storage, preparation, and service of food and beverages at the banquet.
We identified 42 case-patients (25 with confirmed and 17 with suspected cases) and 62 controls; all agreed to participate in the study. Questionnaires were completed between January 24 and March 3, 2011. Twenty-two (51%) case-patients and 59 (97%) controls were from the Dominican Republic (Table 1). Of case-patients, 24 (57%) were wedding guests, 16 (38%) were workers, and 2 (5%) did not attend the reception but ate leftover food from the banquet. Of 62 controls, only 6 (10%) were guests and 56 (90%) were workers (Table 1). Only data from Venezuelan case-patients who received a diagnosis in the Dominican Republic were available for analysis. We did not specify how case-patients were identified.
Figure
Figure. Date and time of onset of cholera cases (N = 40) associated with wedding in La Romana, Dominican Republic, January 2011 (2 case-patients not represented here because time of symptom onset...Odds were higher for case-patients than controls for having consumed cooked shrimp on ice (OR 8.5, 95% CI 3.3–21.71), cooked langostinos (squat lobster) (OR 2.9, 95% CI 1.1–7.7), and beverages with ice cubes (OR 3.6, 95% CI 1.4–9.3), but odds were lower for having consumed mixed rice (OR 0.1, 95% CI 0.01–0.9) or other foods (OR 0.13, 95% CI 0.05–1.03) (Table A1). When these variables were included in a multivariable logistic regression, cooked shrimp on ice (OR 10.8, 95% CI 3.3–35.4), ice cubes (OR 4.1, 95% CI 1.3–13.2), and mixed rice (OR 0.04, 95% CI 0.003–0.5) remained significantly associated with case status (Table 2).
Environmental inspection found that the food was prepared by a caterer outside of the resort. Shrimp and lobster came from the Dominican Republic (Higuey in La Altagracia Province), and the langostinos came from Beata Island in Perdenales Province and Anse-A-Pitre in southeastern Haiti. No samples were available for testing. Inspection of the caterer’s kitchen revealed poor food-handling practices, including improper refrigeration, poor hand hygiene, and nonchlorinated water supply. On the night of the wedding reception, food was set out at 7:00
After a wedding banquet in La Romana Province, Dominican Republic, many persons with gastrointestinal symptoms had laboratory evidence of infection with V. cholerae O1 Ogawa, the cholera serotype identified in the ongoing outbreak on Hispaniola. Cases were positively associated with consumption of cooked shrimp on ice and ice cubes. However, case-patients were not more likely to have eaten shrimp in vinaigrette or shrimp kebab, which may indicate contamination during serving on ice. The treatment of shrimp in vinaigrette with acid (vinegar) and shrimp kebabs with heat may have killed V. cholerae that was present before preparation. Seafood has long been established as a vehicle for cholera transmission (2–5).
Our investigation had several limitations. We were unable to access information from 90% of wedding guests from Venezuela, potentially introducing representation bias. We did not collect information about other meals served to guests and workers before and after the wedding banquet. We did not have itineraries of guests and could not assess whether they had traveled to other parts of Hispaniola affected by cholera before the wedding. We were unable to recruit 2 controls per case-patient, thus reducing the study’s statistical power to detect any weak associations. The international nature of the investigation made it difficult to obtain information in a timely manner, potentially increasing recall bias from widespread media reports of seafood being implicated in the outbreak. Most controls were workers from the Dominican Republic, and most case-patients were guests from Venezuela, which may have led to differential food and drink preferences or differential access to food items on the menu. Moreover, nearly one fifth of case-patients were food handlers, and we cannot exclude the possibility that they cross-contaminated the food or items served. Food and drink served at the reception were unavailable for testing, and we had no information about the source of water used to make ice cubes.
Recommendations to establish a cold chain, use chlorinated water, and exclude ill food handlers were provided to the catering facility and seafood provider. After the investigation, prevention and control measures, including closer adherence to the existing prohibition of importation of high-risk food items from Haiti, were implemented in tourist hotels across the Dominican Republic.
This report highlights the need for international collaboration between public health entities during cholera epidemics. Cholera prevention materials that include information on high-risk food items, such as shellfish and ice should be provided to travelers before they visit potentially cholera-affected areas. Increased awareness and active disease surveillance can help control the spread of cholera outbreaks and prevent secondary transmission.
Our investigation had several limitations. We were unable to access information from 90% of wedding guests from Venezuela, potentially introducing representation bias. We did not collect information about other meals served to guests and workers before and after the wedding banquet. We did not have itineraries of guests and could not assess whether they had traveled to other parts of Hispaniola affected by cholera before the wedding. We were unable to recruit 2 controls per case-patient, thus reducing the study’s statistical power to detect any weak associations. The international nature of the investigation made it difficult to obtain information in a timely manner, potentially increasing recall bias from widespread media reports of seafood being implicated in the outbreak. Most controls were workers from the Dominican Republic, and most case-patients were guests from Venezuela, which may have led to differential food and drink preferences or differential access to food items on the menu. Moreover, nearly one fifth of case-patients were food handlers, and we cannot exclude the possibility that they cross-contaminated the food or items served. Food and drink served at the reception were unavailable for testing, and we had no information about the source of water used to make ice cubes.
Recommendations to establish a cold chain, use chlorinated water, and exclude ill food handlers were provided to the catering facility and seafood provider. After the investigation, prevention and control measures, including closer adherence to the existing prohibition of importation of high-risk food items from Haiti, were implemented in tourist hotels across the Dominican Republic.
This report highlights the need for international collaboration between public health entities during cholera epidemics. Cholera prevention materials that include information on high-risk food items, such as shellfish and ice should be provided to travelers before they visit potentially cholera-affected areas. Increased awareness and active disease surveillance can help control the spread of cholera outbreaks and prevent secondary transmission.
Dr Jiménez is a medical epidemiologist working with the Ministry of Public Health in the Dominican Republic. She recently completed the Central America Field Epidemiology Training Program and is particularly interested in field investigations of communicable disease outbreaks.
Acknowledgments
We thank all persons interviewed during this investigation and our colleagues in the Dominican Republic, United States, Spain, and Venezuela for their assistance.
References
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- Eric D, Mintz TP, Blake PA. Transmission of Vibrio cholerae O1. In: Wachsmuth IK, Orjan Olsvik O, editors. Vibrio cholerae and cholera: molecular to global perspectives. Washington: American Society for Microbiology; 1994. p. 345–56.
- Finelli L, Swerdlow D, Mertz K, Ragazzoni H, Spitalny K. Outbreak of cholera associated with crab brought from an area with epidemic disease. J Infect Dis. 1992;166:1433–5. DOI
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- Wong CS, Ang LW, James L, Goh KT. Epidemiological characteristics of cholera in Singapore, 1992–2007. Ann Acad Med Singapore. 2010;39:507–6.PubMed
- Centers for Disease Control and Prevention. Cholera associated with an international airline flight, 1992. MMWR Morb Mortal Wkly Rep. 1992;41:134–5.PubMed
Figure
Tables
Suggested citation for this article: Jiménez ML, Apostolou A, Palmera Suarez AJ, Meyer L, Hiciano S, Anna Newton A, et al. Multinational cholera outbreak after wedding in the Dominican Republic. Emerg Infect Dis [serial on the Internet]. 2011 Nov [date cited]. http://dx.doi.org/10.3201/eid1711.111263
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