Prevalence of Drug Resistance-Rela... [AIDS Res Hum Retroviruses. 2011] - PubMed - NCBI

AIDS Res Hum Retroviruses. 2011 Sep 18. [Epub ahead of print]
Prevalence of Drug Resistance-Related Polymorphisms in Treatment-naïve Individuals Infected with Non-subtype B HIV-1 in Cameroon.
Fonjungo PN, Youngpairoj AS, Alemnji GA, Eno LT, Lyonga EJ, Eloundou MA, Shanmugam V, Mpoudi-Ngolé E, Kalish ML, Folks TM, Pieniazek D.
Source
Centers for Disease Control and Prevention, CGH/GAP - CDC Ethiopia, P.O Box 1014, Entoto Road, Addis Ababa, Ethiopia, P.O Box 1014, (+251) 11 278 1080, (+251) 11 278 1200; pfonjungo@cdc.gov.

Abstract
Mutations associated with the use of protease (PR) and reverse transcriptase (RT) inhibitors have been mostly mapped for HIV-1 subtype B. The prevalence of these mutations in drug-naïve HIV-1 subtype B infected individuals is low but occurs at high frequencies in treated individuals. To determine the prevalence of treatment-associated mutations in non-B viruses, we analyzed a 1613bp pol region of specimens collected from 57 HIV-1 infected treatment-naïve individuals from Cameroon. Of the 57 HIV-1 sequences, 43 belonged to CRF02-AG, two to CRF11-cpx, six to subtype A, one to subtype D and five were unclassifiable. Of the 57 PR sequences, 100% contained at least one codon change giving substitutions at positions 10, 11, 16, 20, 33, 36, 60, 62, 64, 69, 77, and 89. These substitutions gave the following prevalence pattern, 36I/L (100%, 57/57) > 89M/I (98%, 56/57) > 69K/R (93%, 53/57) > 20I/R (89%, 51/57) > 16E (16%, 9/57) > 64M (12%, 7/57) > 10I (11%, 6/57) > 11V (5%, 3/57) = 62V (5%, 3/57) = 77I (5%, 3/57) > 233F/V (4%, 2/57) = 60E (4%), which differed significantly from subtype B at positions 20, 36, 69 and 89. All but one (98%) of the 57 RT sequences (438 amino acid residues) carried substitutions located at codons 39A (7%), 43E (7%), 122E (7%), 312Q (2%), 333E (2%), 335C/D (89%), 356K (89%), 358K (14%), 365I (2%), 371V (81%), 376S (11%) or 399D (4%); the frequency of these substitutions ranged from <0.5% to 4% in RT of subtype B. The high prevalence of minor mutations associated with protease inhibitors (PI) and reverse transcriptase inhibitors (RTI) represent natural polymorphisms. HIV-1 PR and RT sequences from ARV-naïve HIV-infected persons in Cameroon are important for monitoring the development of resistance to PIs and RTIs as such mutations could lead to treatment failures in individuals undergoing ARV therapy.
PMID:21923557[PubMed - as supplied by publisher]
Prevalence of Drug Resistance-Rela... [AIDS Res Hum Retroviruses. 2011] - PubMed - NCBI