Evaluation of pooling strategies for acute HIV-1 infection screening using nucleic acid amplification testing.

Sullivan TJ, Patel P, Hutchinson A, Ethridge SF, Parker MM.

Source

Wadsworth Center, New York State Department of Health, Albany, NY 12201-2002, USA.

Abstract

A 128-member specimen pooling scheme for acute HIV infection (AHI) detection was evaluated using 21 AHI specimens (range, 1,520 to 500,000 copies/ml) previously identified by RNA testing of 16-member plasma pools. HIV-1 RNA was detectable in 128-member pools for all 21 specimens; however, one pool created from a specimen with 1,827 copies/ml was nonreactive in one of three replicates.

PMID:: 21832020; [PubMed - in process]
PMCID: PMC3187297: [Available on 2012/4/1]

Evaluation of pooling strategies for acute ... [J Clin Microbiol. 2011] - PubMed - NCBI