CIENCIASMEDICASNEWS: Medical News | Medical Articles: Flow Cytometry Cross-matching (FCXM)

viernes, 21 de junio de 2019

Medical News | Medical Articles: Flow Cytometry Cross-matching (FCXM)

	June 21, 2019
	Flow Cytometry
	The latest flow cytometry news from News Medical

Flow Cytometry Cross-matching (FCXM)

FCXM allows for donor antigens and host antibodies to be checked prior to a transplant to avoid a host immune response. The use of a pre-digestion agent can reduce the chances of non-specific binding, which increases the sensitivity and reliability of the analysis.

			A universal FACS-based approach to heterogenous cell sorting A team at the Icahn School of Medicine at Mount Sinai have developed a series of novel fluorescence‐assisted cell sorting (FACS) protocols that enable isolation of neural progenitors, astrocytes, and neurons from virus‐infected cerebral organoids, for downstream multi-omics analysis.

			Imaging single cells travelling at the speed of Usain Bolt The precision and accuracy with which single cells can be analyzed and manipulated has been greatly improved in recent years by the development of microfluidic-based flow cytometry. Despite its sensitivity, microfluidic flow cytometry is limited by low throughput and poor spatial resolution.

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	FACS-based CRISPR screening shows how Chlamydia bacterium invades host cells Researchers at Harvard University are one step closer to understanding how the Chlamydia trachomatis bacterium uses the molecular machinery of host cells for invasion. The team employed fluorescence-activated cell sorting (FACS)-based CRISPR screening for the identification of three major candidate genes known to facilitate pathogenic invasion.

	Virus employs powerful strategy to inhibit natural killer cell function The varicella zoster virus (VZV) dampens the immune system by infecting natural killer cells. However, it was not known how, or if, VZV could directly affect natural killer cell function. Using flow cytometry, scientists now understand that VZV uses a potent strategy to paralyze NK cells.

	UAMS-developed noninvasive device detects melanoma in earliest stages Scientists have developed a non-invasive device that integrates a laser, ultrasound and phone technologies for the detection of circulating tumor cells in the blood. The portable platform is based on a principle called in vivo photoacoustic flow cytometry.

	A novel approach to intraplaque mast cell characterization in atherosclerosis Researchers from Leiden University in the Netherlands have developed a new technique for characterizing intraplaque mast cells; the primary immune mediators of atherosclerotic plaque development.