domingo, 14 de septiembre de 2014

Molecular surveillance of rotavirus infect... [Infect Genet Evol. 2014] - PubMed - NCBI

2014 Sep 2. pii: S1567-1348(14)00311-6. doi: 10.1016/j.meegid.2014.08.023. [Epub ahead of print]

Molecular surveillance of rotavirus infection in Bangui, Central African Republic, October 2011-September 2013.

Banga-Mingo V1, Waku-Kouomou D2, Gody JC3, Esona MD4, Yetimbi JF1, Mbary-Daba R5, Dahl BA6, Dimanche L3, Koyazegbe TD7, Tricou V1, Cavallaro KF6,Guifara G5, Bowen MD4, Gouandjika-Vasilache I1.

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Abstract

BACKGROUND:

The World Health Organization (WHO) recommends the introduction of rotavirus vaccine in the immunization program of all countries. In the Central African Republic (CAR), sentinel surveillance for rotavirus gastroenteritis was established in 2011 by the Ministry of Health, with the support of the Surveillance en Afrique Centrale Project (SURVAC). The purpose of this study was to assess the burden of rotavirus gastroenteritis and to identify rotavirus strains circulating in CAR before the introduction of rotavirus vaccine planned for this year, 2014.

METHODS:

One sentinel site and one laboratory at the national level were designated by the CAR Ministry of Health to participate in this surveillance system. Stool samples were collected from children who met the WHO rotavirus gastroenteritis case definition (WHO, 2006). The samples were first screened for group A rotavirus antigen by enzyme immunoassay (EIA), and genotyping assays performed using a multiplex reverse transcriptase PCR (RT-PCR) technique.

RESULTS:

Between October 2011 and September 2013, 438 stool samples were collected and analyzed for detection of rotavirus antigen; 206 (47%) were positive. Among the 160 (78%) that could be genotyped, G2P[6] was the predominant strain (47%) followed by G1P[8] (25%) and G2P[4] (13%).

CONCLUSIONS:

Almost half of stool samples obtained from children hospitalized with gastroenteritis were positive for rotavirus. These baseline rotavirus surveillance data will be useful to health authorities considering rotavirus vaccine introduction and for evaluating the efficacy of rotavirus vaccine once it is introduced into the routine immunization system.