Absence of high molecular weight proteins 1 and/or 2 is associated with decreased adherence among non-typeable Haemophilus influenzae clinical isolates.

Vuong J, Wang X, Theodore JM, Whitmon J, Gomez de Leon P, Mayer LW, Carlone GM, Romero-Steiner S.

Source

1Meningitis and Vaccine Preventable Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.

Abstract

High molecular weight (Hmw) proteins 1 and 2, type IV pilin protein (PilA), outer-membrane protein P5 (OmpP5), Haemophilus protein D (Hpd) and Haemophilus adhesive protein (Hap) are surface proteins involved in the adherence of non-typeable Haemophilus influenzae. One hundred clinical isolates were evaluated for the presence of the genes encoding these proteins by PCR and for their adherence capacity (AC) to Detroit 562 nasopharyngeal cells (D562). The majority of isolates were from blood (77/100); other sites were also represented. Confluent D562 monolayers (1.2×10(5) cells per well) were inoculated with standardized minimal infective doses (m.o.i.) of 10(2), 10(3) or 10(4) c.f.u. per well. The AC was categorized as low (<10 10c5.="" 21="" 31="" 3d6="" 69="" 72="" absence="" ad6="" adherence.="" adherence:="" adherence="" adhering="" all="" among="" and="" associated="" c.f.u.="" cells.="" cells="" d562="" data="" decreased="" demonstrated="" depending="" detect="" detected="" dot="" evaluated="" expression="" genes="" high-adherence="" high="" hmw1="" hmw1a="" hmw2="" hmw2a="" immunoblots="" in="" increased="" indicate="" isolates.="" isolates="" low="" mabs="" n="69)," of="" on="" or="" our="" p="" per="" percentage="" performed="" presence="" protein="" reacted="" showed="" that="" the="" to="" using="" was="" well.="" were="" while="" with="">