lunes, 11 de noviembre de 2013

Absence of high molecular weight proteins 1 ... [J Med Microbiol. 2013] - PubMed - NCBI

Absence of high molecular weight proteins 1 ... [J Med Microbiol. 2013] - PubMed - NCBI

J Med Microbiol. 2013 Nov;62(Pt 11):1649-56. doi: 10.1099/jmm.0.058222-0. Epub 2013 Aug 29.

Absence of high molecular weight proteins 1 and/or 2 is associated with decreased adherence among non-typeable Haemophilus influenzae clinical isolates.


1Meningitis and Vaccine Preventable Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.


High molecular weight (Hmw) proteins 1 and 2, type IV pilin protein (PilA), outer-membrane protein P5 (OmpP5), Haemophilus protein D (Hpd) and Haemophilus adhesive protein (Hap) are surface proteins involved in the adherence of non-typeable Haemophilus influenzae. One hundred clinical isolates were evaluated for the presence of the genes encoding these proteins by PCR and for their adherence capacity (AC) to Detroit 562 nasopharyngeal cells (D562). The majority of isolates were from blood (77/100); other sites were also represented. Confluent D562 monolayers (1.2×10(5) cells per well) were inoculated with standardized minimal infective doses (m.o.i.) of 10(2), 10(3) or 10(4) c.f.u. per well. The AC was categorized as low (<10 10c5.="" 21="" 31="" 3d6="" 69="" 72="" absence="" ad6="" adherence.="" adherence:="" adherence="" adhering="" all="" among="" and="" associated="" c.f.u.="" cells.="" cells="" d562="" data="" decreased="" demonstrated="" depending="" detect="" detected="" dot="" evaluated="" expression="" genes="" high-adherence="" high="" hmw1="" hmw1a="" hmw2="" hmw2a="" immunoblots="" in="" increased="" indicate="" isolates.="" isolates="" low="" mabs="" n="69)," of="" on="" or="" our="" p="" per="" percentage="" performed="" presence="" protein="" reacted="" showed="" that="" the="" to="" using="" was="" well.="" were="" while="" with="">


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