Influenza B Viruses with Mutation in the Neuraminidase Active Site, North Carolina, USA, 2010–11 - Vol. 17 No. 11 - November 2011 - Emerging Infectious Disease journal - CDC

Volume 17, Number 11—November 2011

Dispatch

Influenza B Viruses with Mutation in the Neuraminidase Active Site, North Carolina, USA, 2010–11

Article Contents

• The Study
• Conclusions
• Acknowledgments
• References
• Figure
• Table 1
• Table 2
• Suggested Citation

Katrina Sleeman, Tiffany G. Sheu, Zack Moore, Susan Kilpatrick, Shikha Garg, Alicia M. Fry, and Larisa V. Gubareva 

Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (K. Sleeman, T.G. Sheu, S. Garg, A.M. Fry, L.V. Gubareva); Battelle, Atlanta (T.G. Sheu); North Carolina Department of Health and Human Services, Raleigh, North Carolina, USA (Z. Moore, S. Kilpatrick)

Suggested citation for this article

Abstract

Oseltamivir is 1 of 2 antiviral medications available for the treatment of influenza B virus infections. We describe and characterize a cluster of influenza B viruses circulating in North Carolina with a mutation in the neuraminidase active site that may reduce susceptibility to oseltamivir and the investigational drug peramivir but not to zanamivir.

Influenza B viruses are responsible for sporadic seasonal influenza illness and can be associated with severe illness and death. In the United States, there are 2 classes of antiviral drugs licensed by the Food and Drug Administration for treatment of influenza infections. The adamantanes are ineffective against influenza B viruses, which limits the available antiviral options to 2 neuraminidase inhibitors (NAIs), inhaled zanamivir and oral oseltamivir. Influenza B viruses seem to have reduced susceptibility to NAIs compared with influenza A viruses on the basis of neuraminidase inhibition (NAI) assays (1,2). Furthermore, in clinical studies, changes conferring either resistance or reduced susceptibility to NAIs have been identified in the neuraminidase (NA) of influenza B viruses isolated from patients after treatment (3–6). Although the use of an antiviral agent can lead to the development of drug resistance, influenza B viruses with a reduced NAI susceptibility have also been recovered from patients with no history of exposure (5,7–10). It is therefore plausible that such mutations may be naturally occurring within the NA of influenza B viruses.

The Study

During routine influenza antiviral susceptibility surveillance, an influenza B virus, B/North Carolina/11/2010, with reduced susceptibility to oseltamivir and the investigational NAI peramivir was detected by using the fluorescent NAI assay based on IC₅₀ values (amount of NAI required to inhibit 50% of viral NA activity). According to the current algorithm, viruses with elevated IC₅₀ values, when compared with a drug-susceptible control reference virus, are further investigated by using either conventional sequencing or pyrosequencing. Sequence analysis for the NA gene of B/North Carolina/11/2010 showed a novel substitution, present as a mixed population, of isoleucine (I) to valine (V) at position 221 (B NA numbering corresponds to 222 in N2 NA amino acid numbering). A substitution of I to threonine (T) at 221 has previously been associated with reduced susceptibility to NAIs in influenza B viruses (1,5,9). Moreover, reduced susceptibility to oseltamivir has been reported in viruses with variation at the corresponding residue (223, N1 NA numbering) in the pandemic (H1N1) 2009 virus (11,12) and in influenza A/H5N1 (13) and A/H3N2 viruses (14).
Subsequent fluorescent NAI testing of isolates recovered during surveillance showed a cluster of 14 influenza B viruses from North Carolina with elevated oseltamivir IC₅₀ values compared with reference wild-type influenza B, wild-type pandemic (H1N1) 2009, and wild-type A(H3N2) viruses; a similar trend was observed for peramivir IC₅₀ values (Table 1). When comparing the pandemic (H1N1) 2009 virus with the oseltamivir-resistance conferring H275Y substitution and an influenza A (H3N2) virus with the oseltamivir-resistance conferring E119V substitution, the North Carolina B viruses showed intermediate susceptibility (Table 1). The influenza B virus carrying the R152K substitution was resistant to all NAIs compared with the influenza B viruses with I221V (Table 1). In the chemiluminescent NAI assay, the oseltamivir IC₅₀ values for the I221V variants were greater than that for the E119V influenza A (H3N2) virus variant, which has been associated with oseltamivir resistance (Table 1) (15). Pyrosequencing analysis showed I221V as well as wild-type (I221) in the propagated viruses used in the NAI assays. The presence of wild-type variants is likely to reduce IC₅₀ values.

A total of 258 influenza B virus isolates from domestic and foreign laboratories submitted to the Centers for Disease Control and Prevention for routine surveillance were screened for the I221V substitution by using single-nucleotide polymorphism (SNP) pyrosequencing analysis (10). All viruses were wild type at this position, with the exception of the 14 viruses from North Carolina with reduced susceptibility in the NAI assay (Table 2). All 14 viruses were collected from patients in North Carolina during November 2010 through February 2011.

Because some susceptibility-altering NA mutations have been shown to arise from virus propagation in tissue culture (15), pyrosequencing analysis at position 221 in available matching clinical specimens was performed to rule out cell culture selection. The I221V substitution was identified in the 9 available matching clinical specimens (Table 2). Notably, most of the clinical specimens contained higher percentages of the V221 variant compared with the matching virus isolates, which may indicate a potential selective pressure for the wild-type variant (I221) in cell culture.
An epidemiologic investigation and enhanced surveillance was initiated in cooperation with the North Carolina Department of Health and Human Services. Of 220 patients with influenza B virus infections in North Carolina during November 2010 through March 2011, specimens from 209 patients underwent pyrosequencing analysis. Specimens from 45 (22%) patients from 13 counties contained the I221V mutation based on SNP pyrosequencing analysis; patient median age was 12 years (range 6 months–60 years). Among 199 patients with available antiviral treatment information, specifically for oseltamivir use, none had documented exposure to the virus before specimen collection. This finding may indicate that influenza B viruses carrying the I221V mutation are co-circulating with wild-type influenza B viruses in North Carolina.

Conclusions

Although the NA change I221V has been seen among the N1 NA subtype of influenza A viruses (1,13), such a change has not been reported in influenza B viruses. Amino acid 221 is known to be a highly conserved residue of the NA enzyme active site. To date, all influenza B viruses with the I221V substitution appear to be limited geographically; however, monitoring is ongoing. Although oseltamivir IC₅₀ values obtained with the influenza B viruses carrying the I221V substitution are similar to those seen with influenza A(H3N2) viruses carrying the oseltamivir-resistance conferring substitution E119V (Table 1), the clinical significance of the altered susceptibility associated with I221V in influenza B viruses is unknown at this time and warrants further investigation. Furthermore, such variant-dependent elevated IC₅₀ values highlight the need for establishing a correlation between laboratory-determined IC₅₀ values and clinical resistance.

Figure

Figure. Phylogenetic analysis of A) hemagglutinin and B) neuraminidase genes of Victoria lineage type B influenza viruses (n = 89). Red indicates the 2010−2011 Northern Hemisphere vaccine strain; blue indicates the cluster...

Phylogenetic analysis of the hemagglutinin gene of the North Carolina B viruses carrying the I221V change in the NA is consistent with the B Victoria lineage (Figure, panel A). Similarly, phylogenetic analysis of the NA gene demonstrated that the North Carolina B viruses with the I221V change also belong to the B Victoria lineage and form a cluster because of the I221V substitution (Figure, panel B). As of March 2011, of the 438 influenza B viruses isolated in the United States, 94% were antigenically characterized as B/Brisbane/60/2008-like (B-Victoria lineage) (www.cdc.gov/flu/weekly). The cluster of North Carolina influenza B viruses carrying the I221V mutation antigenically matched the current influenza B component of the seasonal influenza vaccine. Data collected from an ongoing epidemiologic and clinical correlation study will be the subject of a more detailed future report.

Dr Sleeman is an associate service fellow in the Molecular Epidemiology Team of the Influenza Division at the Centers for Disease Control and Prevention in Atlanta, Georgia. Her research interests are negative-strand RNA viruses and antiviral drugs, with a particular emphasis on influenza viruses and antiviral drug resistance.

Acknowledgments

We thank all collaborators in the World Health Organization Global Influenza Surveillance Network, including the National Influenza Centers, for submission of isolates. We also thank members of the Molecular Epidemiology Team, the Virus Reference Team, and the Sequencing Group of the Influenza Division at the Centers for Disease Control and Prevention for contributions with this project.

References

1. Monto  AS, McKimm-Breschkin  JL, Macken  C, Hampson  AW, Hay  A, Klimov  A, Detection of influenza viruses resistant to neuraminidase inhibitors in global surveillance during the first 3 years of their use. Antimicrob Agents Chemother. 2006;50:2395–402. DOIPubMed
2. Carr  S, Ilyushina  NA, Franks  J, Adderson  EE, Caniza  M, Govorkova  EA, Oseltamivir-resistant influenza A and B viruses pre- and post-viral therapy in children and young adults with cancer. Pediatr Infect Dis J. 2011;30:284–8. DOIPubMed
3. Kawai  N, Ikematsu  H, Iwaki  N, Maeda  T, Satoh  I, Hirotsu  N, A comparison of the effectiveness of oseltamivir for the treatment of influenza A and influenza B: a Japanese multicenter study of the 2003–2004 and 2004–2005 influenza seasons. Clin Infect Dis. 2006;43:439–44. DOIPubMed
4. Gubareva  LV, Matrosovich  MN, Brenner  MK, Bethell  RC, Webster  RG. Evidence for zanamivir resistance in an immunocompromised child infected with influenza B virus. J Infect Dis. 1998;178:1257–62. DOIPubMed
5. Hatakeyama  S, Sugaya  N, Ito  M, Yamazaki  M, Ichikawa  M, Kimura  K, Emergence of influenza B viruses with reduced sensitivity to neuraminidase inhibitors. JAMA. 2007;297:1435–42. DOIPubMed
6. Sugaya  N, Tamura  D, Yamazaki  M, Ichikawa  M, Kawakami  C, Kawaoka  Y, Comparison of the clinical effectiveness of oseltamivir and zanamivir against influenza infection in children. Clin Infect Dis. 2008;47:339–45. DOIPubMed
7. Hurt  AC, Kimm-Breschkin  JL, McDonald  M, Barr  IG, Komadina  N, Hampson  AW. Identification of a human influenza type B strain with reduced sensitivity to neuraminidase inhibitor drugs. Virus Res. 2004;103:205–11. DOIPubMed
8. Hurt  AC, Iannello  P, Jachno  K, Komadina  N, Hampson  AW, Barr  IG, Neuraminidase inhibitor-resistant and -sensitive influenza B viruses isolated from an untreated human patient. Antimicrob Agents Chemother. 2006;50:1872–4. DOIPubMed
9. Sheu  TG, Deyde  VM, Okomo-Adhiambo  M, Garten  RJ, Xu  X, Bright  RA, Surveillance for neuraminidase inhibitor resistance among human influenza A and B viruses circulating worldwide from 2004 to 2008. Antimicrob Agents Chemother. 2008;52:3284–92. DOIPubMed
10. Sheu  TG, Deyde  VM, Garten  RJ, Klimov  AI, Gubareva  LV. Detection of antiviral resistance and genetic lineage markers in influenza B virus neuraminidase using pyrosequencing. Antiviral Res. 2010;85:354–60. DOIPubMed
11. Nguyen  HT, Fry  AM, Loveless  PA, Klimov  AI, Gubareva  LV. Recovery of a multidrug-resistant strain of pandemic influenza A 2009 (H1N1) virus carrying a dual H275Y/I223R mutation from a child after prolonged treatment with oseltamivir. Clin Infect Dis. 2010;51:983–4. DOIPubMed
12. van der Vries  E, Stelma  FF, Boucher  CAB. Emergence of a multidrug-resistant pandemic influenza A(H1N1) virus. N Engl J Med. 2010;363:1381–2. DOIPubMed
13. Hurt  AC, Holien  JK, Barr  IG. In vitro generation of neuraminidase inhibitor resistance in A(H5N1) influenza viruses. Antimicrob Agents Chemother. 2009;53:4433–40. DOIPubMed
14. Baz  M, Abed  Y, McDonald  J, Boivin  G. Characterization of a multidrug-resistant influenza A/H3N2 virus shed during 1 year by an immunocompromised child. Clin Infect Dis. 2006;43:1555–61. DOIPubMed
15. Okomo-Adhiambo  M, Demmler-Harrison  GJ, Deyde  VM, Sheu  TG, Xu  X, Klimov  AI, Detection of E119V and E119I mutations in influenza A (H3N2) viruses isolated from an immunocompromised patient: challenges in diagnosis of oseltamivir resistance. Antimicrob Agents Chemother. 2010;54:1834–41. DOIPubMed

Figure

• Figure. Phylogenetic analysis of A) hemagglutinin and B) neuraminidase genes of Victoria lineage type B influenza viruses (n = 89). Red indicates the 2010−2011 Northern Hemisphere vaccine strain; blue indicates the...

Tables

• Table 1. Comparison of influenza virus susceptibility, North Carolina, USA, 2010−11 influenza seasons
• Table 2. Percentage composition of isoleucine and valine at position 221 in the neuraminidase of virus isolates and clinical specimens with reduced susceptibility to oseltamivir and peramivir, North Carolina, USA, 2010−11 influenza season...

Suggested citation for this article: Sleeman K, Sheu TG, Moore Z, Kilpatrick S, Garg S, Fry AM, et al. Influenza B viruses with mutation in the neuraminidase active site, North Carolina, USA, 2010−11. Emerg Infect Dis [serial on the Internet]. 2011 Nov [date cited]. http://dx.doi.org/10.3201/eid1711.110787

DOI: 10.3201/eid1711.110787

Influenza B Viruses with Mutation in the Neuraminidase Active Site, North Carolina, USA, 2010–11 - Vol. 17 No. 11 - November 2011 - Emerging Infectious Disease journal - CDC