domingo, 30 de diciembre de 2018

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Journal of Ecology and Environment | Home

Journal of Ecology and Environment | Home

Journal of Ecology and Environment



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  • ​​​​​​​Journal of Ecology and Environment is the official journal of the Ecological Society of Korea.
    The journal was founded in 1977 and transferred to BioMed Central in June 2016. An electronic archive of articles published between 1977 and March 2016 can be found here.




Lipids in Health and Disease | Home page

Lipids in Health and Disease | Home page

Lipids in Health and Disease

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Construction of a high-density genetic map: genotyping by sequencing (GBS) to map purple seed coat color (Psc) in hulless barley | Hereditas | Full Text

Construction of a high-density genetic map: genotyping by sequencing (GBS) to map purple seed coat color (Psc) in hulless barley | Hereditas | Full Text

The phenotype pictures of female, male and four types of seed coat color.

Hereditas

Construction of a high-density genetic map: genotyping by sequencing (GBS) to map purple seed coat color (Psc) in hulless barley

Hereditas2018155:37
  • Received: 2 July 2018
  • Accepted: 18 October 2018
  • Published: 

Abstract

Background

Colored hulless barley are more suitable in food processing compared to normal (yellow) varieties because it is rich in bioactive compounds and produces higher extraction pearling fractions. Therefore, seed coat color is an important agronomic trait for the breeding and study of hulless barley.

Results

Genotyping-by-sequencing single-nucleotide polymorphism (GBS-SNP) analysis of a doubled haploid (DH) mapping population (Nierumuzha × Kunlun10) was conducted to map the purple seed coat color genes (Psc). A high-density genetic map of hulless barley was constructed, which contains 3662 efficient SNP markers with 1129 bin markers. Seven linkage groups were resolved, which had a total length of 645.56 cM. Chromosome length ranged from 60.21 cM to 127.21 cM, with average marker density of 0.57 cM. A total of five loci accounting for 3.79% to 23.86% of the observed phenotypic variation for Psc were detected using this high-density map. Five structural candidate genes (F3’MHIDUF3GTUFGT and 5MAT) and one regulatory factor (Ant1) related to flavonoid or anthocyanin biosynthesis were identified..

Conclusions

Five structural candidate genes and one regulatory factor related to flavonoid or anthocyanin biosynthesis have been identified using a high-density genetic map of hulless barley. This study lays the foundation for map-based cloning of Psc but provides a valuable tool for studying marker-trait associations and its application to marker-assisted breeding of hulless barley.

Keywords

  • Hulless barley·genotyping-by-sequencing (GBS)
  • Purple seed coat color (Psc)
  • Linkage analysis

Hereditas | Home page

Hereditas | Home page

Hereditas

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Genome Biology | Home page

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Focus: Genome Editing

Read the latest Reviews on genome editing across BMC flagship journals.

Genome editing for inborn errors of metabolism: advancing towards the clinic
Jessica L. Schneller, Ciaran M. Lee, Gang Bao and Charles P. Venditti
Published in BMC Medicine on: 27 February 2017
Structural insights into the inactivation of CRISPR-Cas systems by diverse anti-CRISPR proteins
Yuwei Zhu, Fan Zhang and Zhiwei Huang
Published in BMC Biology on: 19 March 2018
Resources for the design of CRISPR gene editing experiments
Daniel B. Graham and David E. Root
Published in Genome Biology on: 27 November 2015
Key challenges in bringing CRISPR-mediated somatic cell therapy into the clinic
Dianne Nicol, Lisa Eckstein, Michael Morrison, Jacob S. Sherkow, Margaret Otlowski, Tess Whitton, Tania Bubela, Kathryn P. Burdon, Don Chalmers, Sarah Chan, Jac Charlesworth, Christine Critchley, Merlin Crossley, Sheryl de Lacey, Joanne L. Dickinson, Alex W. Hewitt, Joanne Kamens, Kazuto Kato, Erika Kleiderman, Satoshi Kodama, John Liddicoat, David A. Mackey, Ainsley J. Newson, Jane Nielsen, Jennifer K. Wagner and Rebekah E. McWhirter
Published in Genome Medicine on: 25 September 2017

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Latest articles from Genome Medicine