Porcine Rotavirus Related to Human Rotaviruses | CDC EID > Volume 17, Number 8–August 2011

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Volume 17, Number 8–August 2011
Dispatch
Porcine Rotavirus Closely Related to Novel Group of Human Rotaviruses

Mitsutaka Wakuda, Tomihiko Ide, Jun Sasaki, Satoshi Komoto, Junichi Ishii, Takeshi Sanekata, and Koki Taniguchi Comments to Author
Author affiliations: Fujita Health University School of Medicine, Toyoake, Japan (M. Wakuda. T. Ide, J. Sasaki, S. Komoto, J. Ishii, K. Taniguchi); and Tottori University, Tottori, Japan (T. Sanekata)

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Abstract
We determined nucleotide sequences and inferred amino acid sequences of viral protein (VP) 4, VP6, VP7, and nonstructural protein 4 genes of a porcine rotavirus strain (SKA-1) from Japan. The strain was closely related to a novel group of human rotavirus strains (B219 and J19).

Rotaviruses, a member of family Reoviridae, are a major etiologic agent of acute gastroenteritis in humans and animals worldwide. Rotaviruses are classified into 7 groups designated A–G (1–3). Group A rotaviruses cause severe diarrhea in infants and children, and is estimated to be associated with 527,000 childhood deaths annually. They are also responsible for diarrhea in young mammals and birds of various species. Group B rotaviruses were first detected in a large water-borne outbreak of diarrhea among adults in the People's Republic of China, and were recently found in Bangladesh, India, and Myanmar. They have also been detected in cows, pigs, and rats. Group C rotaviruses cause sporadic and epidemic gastroenteritis in children and adults. They have also been detected in pigs, cows, and other animals. Group E rotaviruses were detected in pigs, and group D, F, and G rotaviruses were detected in chickens.

The complete nucleotide sequence of the genome of an avian group D rotavirus strain has been reported (4). However, little information on rotavirus groups E–G has been reported (1–3,5,6).

Human rotavirus strains J19 and B219, which are not classified into group A, B, or C, have been detected in China and Bangladesh (7–11). Strain J19, which was detected during a large epidemic of diarrhea in adults in China in 1997, has been propagated in human embryo kidney cells (7,8). Complete nucleotide sequences of all 11 RNA segments of strain J19 have been determined (9). In addition, the complete nucleotide sequence of the genome of Bangladesh strain B219 has been determined (10,11). Comparative sequence analysis showed that these 2 rotavirus strains are part of a novel group of rotaviruses.

We determined complete nucleotide sequences of the 4 RNA segments encoding viral protein 4 (VP4), VP6, VP7, and nonstructural protein 4 (NSP4) of a porcine rotavirus strain (SKA-1) from Japan (12) by using cDNA products obtained by a single-primer amplification method (13,14). Sequence data showed that SKA-1 is closely related to the novel group of human rotaviruses (J19 and B219).

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Suggested Citation for this Article

Wakuda M, Ide T, Sasaki J, Komoto S, Ishii J, Sanekata T, et al. Porcine rotavirus closely related to novel group of human rotaviruses. Emerg Infect Dis [serial on the Internet]. 2011 Aug [date cited]. http://www.cdc.gov/EID/content/17/8/101466.htm

DOI: 10.3201/eid1708.101466

Comments to the Authors

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Koki Taniguchi, Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan; email: kokitani@fujita-hu.ac.jp