viernes, 16 de julio de 2010

Abstract | Transcriptomic epidemiology of smoking: the effect of smoking on gene expression in lymphocytes


Transcriptomic epidemiology of smoking: the effect of smoking on gene expression in lymphocytes
Jac C Charlesworth , Joanne E Curran , Matthew P Johnson , Harald HH Goring , Thomas D Dyer , Vincent P Diego , Jack W Kent Jr , Micheal C Mahaney , Laura Almasy , Jean W MacCluer , Eric K Moses and John Blangero

BMC Medical Genomics 2010, 3:29doi:10.1186/1755-8794-3-29



Published: 15 July 2010

Abstract (provisional)

Background
This investigation offers insights into system-wide pathological processes induced in response to cigarette smoke exposure by determining its influences at the gene expression level.

Methods
We obtained genome-wide quantitative transcriptional profiles from 1,240 individuals from the San Antonio Family Heart Study, including 297 current smokers. Using lymphocyte samples, we identified 20,413 transcripts with significantly detectable expression levels, including both known and predicted genes. Correlation between smoking and gene expression levels was determined using a regression model that allows for residual genetic effects.

Results
With a conservative false-discovery rate of 5% we identified 323 unique genes (342 transcripts) whose expression levels were significantly correlated with smoking behavior. These genes showed significant over-representation within a range of functional categories that correspond well with known smoking-related pathologies, including immune response, cell death, cancer, natural killer cell signaling and xenobiotic metabolism.

Conclusions
Our results indicate that not only individual genes but entire networks of gene interaction are influenced by cigarette smoking. This is the largest in vivo transcriptomic epidemiological study of smoking to date and reveals the significant and comprehensive influence of cigarette smoke, as an environmental variable, on the expression of genes. The central importance of this manuscript is to provide a summary of the relationships between gene expression and smoking in this exceptionally large cross-sectional data set.

The complete article is available as a provisional PDF. The fully formatted PDF and HTML versions are in production.
open here please:
Abstract | Transcriptomic epidemiology of smoking: the effect of smoking on gene expression in lymphocytes

No hay comentarios:

Publicar un comentario